Abstract：
The β-glucuronidase (GUS) reporter gene system is an important technique with versatile uses in the study of flower development in a broad range of species. Transcriptional and translational GUS fusions are used to characterize gene and protein expression patterns, respectively, during reproductive development. Additionally, GUS reporters can be used to map cis-regulatory elements within promoter sequences and to investigate whether genes are regulated post-transcriptionally. Gene trap/enhancer trap GUS constructs can be used to identify novel genes involved in flower development and marker lines useful in mutant characterization. Flower development studies primarily have used the histochemical assay in which inflorescence tissue from transgenic plants containing GUS reporter genes are stained for GUS activity and examined as whole-mounts or subsequently embedded into wax and examined as tissue sections. In addition, quantitative GUS activity assays can be performed on either floral extracts or intact flowers using a fluorogenic GUS substrate. Another use of GUS reporters is as a screenable marker for plant transformation. A simplified histochemical GUS assay can be used to quickly identify transgenic tissues.
摘要：
β-葡糖醛酸糖苷酶（GUS）报告基因系统是一种重要的技术，在研究各种物种的花卉发育中具有广泛的用途。转录和翻译GUS融合用于表征基因和蛋白质表达模式，分别,在生殖发育过程中。此外,GUS报告基因可用于定位启动子序列内的顺式调节元件，并研究基因是否在转录后受到调节。基因捕获/增强子捕获GUS构建体可用于鉴定参与花发育的新基因和用于突变体表征的标记系。花发育研究主要使用组织化学测定，其中对含有GUS报告基因的转基因植物的花序组织进行GUS活性染色，并作为整体进行检查或随后嵌入蜡中并作为组织切片进行检查。此外,可以使用荧光GUS底物对花提取物或完整的花进行定量GUS活性测定。GUS报告基因的另一个用途是作为植物转化的筛选标记。简化的组织化学GUS测定可用于快速鉴定转基因组织。
