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Abstract：
UNASSIGNED: A specific type of mesenchymal stem/progenitor cells (MSPCs), CD105+ is reported to aid in cartilage regeneration through TGF-β/Smad2-signalling. The purpose of this study was to identify and characterize CD105+ MSPCs in temporomandibular joint (TMJ) cartilage.
UNASSIGNED: MSPCs were isolated from mouse TMJ condyle explants and evaluated for their clonogenicity and pluripotential abilities. MSPC were examined for CD105 antigen using immunohistochemistry and flow cytometry.
UNASSIGNED: Immunohistochemistry revealed presence of CD105+ MSPCs in the proliferative zone of condyle\'s cartilage. Only 0.2% of isolated MSPCs exhibited CD105, along with the stem cell surface markers CD44 and Sca-1. In CD105+ MSPCs, intracellular immunostaining revealed significantly higher (p < 0.05) protein levels of collagen type 1, 2, proteoglycan 4. Ability for chondrogenic differentiation was found to be significantly higher (p < 0.05) after 4 weeks compared to CD105- cells, using alcian blue staining. CD105+ cells were found to resemble an early MSPC subgroup with significantly higher gene expression of biglycan, proteoglycan 4, collagen type 2, Gli2, Sox5 (p < 0.001) and Sox9 (p < 0.05). In contrast, significantly lower levels of Runx2 (p < 0.05), Osterix, Trps1, Col10a1 (p < 0.01), Ihh (p < 0.001) related to chondrocyte senescence and commitment to osteogenic lineage, were observed compared to CD105- cells.
UNASSIGNED: The study showed the existence of a CD105+ MSPC subgroup within TMJ fibrocartilage that may be activated to aid in fibrocartilage repair.
UNASSIGNED: MSPCs were isolated from mouse TMJ condyle explants and evaluated for their clonogenicity and pluripotential abilities. MSPC were examined for CD105 antigen using immunohistochemistry and flow cytometry.
UNASSIGNED: Immunohistochemistry revealed presence of CD105+ MSPCs in the proliferative zone of condyle\'s cartilage. Only 0.2% of isolated MSPCs exhibited CD105, along with the stem cell surface markers CD44 and Sca-1. In CD105+ MSPCs, intracellular immunostaining revealed significantly higher (p < 0.05) protein levels of collagen type 1, 2, proteoglycan 4. Ability for chondrogenic differentiation was found to be significantly higher (p < 0.05) after 4 weeks compared to CD105- cells, using alcian blue staining. CD105+ cells were found to resemble an early MSPC subgroup with significantly higher gene expression of biglycan, proteoglycan 4, collagen type 2, Gli2, Sox5 (p < 0.001) and Sox9 (p < 0.05). In contrast, significantly lower levels of Runx2 (p < 0.05), Osterix, Trps1, Col10a1 (p < 0.01), Ihh (p < 0.001) related to chondrocyte senescence and commitment to osteogenic lineage, were observed compared to CD105- cells.
UNASSIGNED: The study showed the existence of a CD105+ MSPC subgroup within TMJ fibrocartilage that may be activated to aid in fibrocartilage repair.
摘要：
■一种特定类型的间充质干细胞/祖细胞（MSPCs），据报道,CD105+通过TGF-β/Smad2-信号传导辅助软骨再生。这项研究的目的是鉴定和表征颞下颌关节（TMJ）软骨中的CD105MSPC。
■从小鼠TMJ髁突外植体中分离出MSPC，并评估其克隆形成能力和多潜能能力。使用免疫组织化学和流式细胞术检查MSPC的CD105抗原。
■免疫组织化学显示在髁突软骨的增殖区中存在CD105+MSPCs。只有0.2％的分离的MSPC表现出CD105，以及干细胞表面标志物CD44和Sca-1。在CD105+MSPC中，细胞内免疫染色显示胶原蛋白1、2、蛋白聚糖4的蛋白质水平明显更高（p<0.05）。与CD105-细胞相比，4周后发现软骨形成分化的能力显着更高（p<0.05）。使用阿尔辛蓝染色。发现CD105细胞类似于早期MSPC亚群，其双糖链蛋白聚糖的基因表达明显更高，蛋白聚糖4、胶原2型、Gli2、Sox5(p<0.001)和Sox9(p<0.05)。相比之下,Runx2的水平显着降低（p<0.05），Osterix,Trps1,Col10a1(p<0.01),Ihh（p<0.001）与软骨细胞衰老和成骨谱系有关，与CD105-细胞相比观察到。
■研究表明，TMJ纤维软骨中存在CD105+MSPC亚群，可被激活以帮助纤维软骨修复。
■从小鼠TMJ髁突外植体中分离出MSPC，并评估其克隆形成能力和多潜能能力。使用免疫组织化学和流式细胞术检查MSPC的CD105抗原。
■免疫组织化学显示在髁突软骨的增殖区中存在CD105+MSPCs。只有0.2％的分离的MSPC表现出CD105，以及干细胞表面标志物CD44和Sca-1。在CD105+MSPC中，细胞内免疫染色显示胶原蛋白1、2、蛋白聚糖4的蛋白质水平明显更高（p<0.05）。与CD105-细胞相比，4周后发现软骨形成分化的能力显着更高（p<0.05）。使用阿尔辛蓝染色。发现CD105细胞类似于早期MSPC亚群，其双糖链蛋白聚糖的基因表达明显更高，蛋白聚糖4、胶原2型、Gli2、Sox5(p<0.001)和Sox9(p<0.05)。相比之下,Runx2的水平显着降低（p<0.05），Osterix,Trps1,Col10a1(p<0.01),Ihh（p<0.001）与软骨细胞衰老和成骨谱系有关，与CD105-细胞相比观察到。
■研究表明，TMJ纤维软骨中存在CD105+MSPC亚群，可被激活以帮助纤维软骨修复。
