Abstract：
Sugarcane (Saccharum hybrid) is an important cash crop grown in tropical and subtropical countries. Ratoon stunting disease (RSD), caused by a xylem-inhabiting bacterium, Leifsonia xyli subsp. xyli (Lxx) is one of the most economically significant diseases globally. RSD results in severe yield losses because its highly contagious nature and lack of visually identifiable symptoms make it harder to devise an effective management strategy. The efficacy of current management practices is hindered by implementation difficulties caused by lack of resources, high cost, and difficulties in monitoring. Rapid detection of the causal pathogen in vegetative planting material is crucial for sugarcane growers to manage this disease. Several microscopic, serological, and molecular-based methods have been developed and used for detecting the RSD pathogen. Although these methods have been used across the sugarcane industry worldwide to diagnose Lxx, some lack reliability or specificity, are expensive and time-consuming to apply, and most of all, are not suitable for on-farm diagnosis. In recent decades, there has been significant progress in the development of integrated isothermal amplification-based microdevices for accurate human and plant pathogen detection. There is a significant opportunity to develop a novel diagnostic method that integrates nanobiosensing with isothermal amplification within a microdevice format for accurate Lxx detection. In this review, we summarize (i) the historical background and current knowledge of sugarcane ratoon stunting disease, including some aspects related to transmission, pathosystem, and management practices; and (ii) the drawbacks of current diagnostic methods and the potential for application of advanced diagnostics to improve disease management.
摘要：
甘蔗（甘蔗杂种）是热带和亚热带国家种植的重要经济作物。宿根发育迟缓症(RSD),由木质部居住的细菌Leifsoniaxyli亚种引起。xyli(Lxx)，是全球最具经济意义的。RSD由于其高度传染性而导致严重的产量损失，缺乏视觉识别的症状，使制定有效的管理战略变得更加困难。由于缺乏资源，实施困难阻碍了当前管理做法的有效性，高成本,和监测方面的困难。快速检测植物种植材料中的病原体对于甘蔗种植者控制这种疾病至关重要。几个微观的，血清学,和基于分子的方法已经被开发并用于检测RSD病原体。尽管这些方法已在全球甘蔗行业中用于诊断Lxx，有些缺乏可靠性或特异性，是昂贵的，申请耗时，最重要的是，不适合农场诊断。近几十年来,在开发用于准确检测人类和植物病原体的基于等温扩增的集成微设备方面取得了重大进展。存在开发新型诊断方法的重要机会，该方法将纳米生物传感与等温扩增集成在微型设备格式中以进行精确的Lxx检测。在这次审查中,我们总结(一)甘蔗宿根矮化病的历史背景和当前知识，包括与传输相关的一些方面，病态系统，和管理实践；（ii）当前诊断方法的缺点和应用高级诊断技术以改善疾病管理的潜力。
