PDF(Pubmed)
Abstract：
Astragalus membranaceus (A. membranaceus) is a homologous plant with high medicinal and edible value. Therefore, the extraction methods of Astragalus polysaccharide (APS) have attracted the attention of many research groups, but the yield of the active components is still not high. The aim of this study was to extract APS by a semi-bionic extraction method, optimize the extraction process, and evaluate the anti-aging activities of APS in vivo. The results showed that the APS yield was 18.23% when extracted by the semi-bionic extraction method. Anti-aging evaluation in rats showed that APS extracted by this method significantly decreased the malondialdehyde (MDA) content and increased superoxide dismutase (SOD) activity to cope with D-galactose-induced aging. Serum metabolomic analysis indicated that a total of 48 potential biomarkers showed significant differences, mainly involving 5 metabolic pathways. These altered metabolic pathways were mainly related to energy metabolism, amino acid metabolism, and lipid metabolism. These results indicated that the semi-bionic extraction method can effectively improve the yield of APS, and the extracted APS exhibited anti-aging activity in rats. Our study provided a novel and effective method to extract APS and indicated that APS can be used as functional food and natural medicine to delay aging and prevent its complications.
摘要：
黄芪（A.膜虫）是一种具有很高药用和食用价值的同源植物。因此,黄芪多糖(APS)的提取方法引起了许多研究小组的关注,但活性成分的收率仍然不高。本研究的目的是通过半仿生提取方法提取APS，优化提取工艺,并评价APS的体内抗衰老活性。结果表明,半仿生提取法提取的APS得率为18.23%。对大鼠的抗衰老评估表明，通过该方法提取的APS显着降低了丙二醛（MDA）含量，增加了超氧化物歧化酶（SOD）活性，以应对D-半乳糖引起的衰老。血清代谢组学分析表明，共有48个潜在的生物标志物显示出显著差异，主要涉及5个代谢途径。这些改变的代谢途径主要与能量代谢有关,氨基酸代谢，和脂质代谢。结果表明半仿生提取法能有效提高APS的得率,提取的APS对大鼠具有抗衰老活性。本研究为APS的提取提供了一种新的有效方法，表明APS可作为功能性食品和天然药物，延缓衰老，预防其并发症。
