Abstract：
Wheat leaf rust (Lr), which is caused by Puccinia triticina Eriks. (Pt), is one of the most important wheat diseases affecting wheat production globally. Using resistant wheat cultivars is the most economical and environmentally friendly way to control leaf rust. The Italian wheat cultivar Libellula has demonstrated good resistance to Lr in field studies. To identify the genetic basis of Lr resistance in \'Libellula\', 248 F6 recombinant inbred lines from the cross \'Libellula\'/\'Huixianhong\' was phenotyped for Lr severity in seven environments: the 2014/2015, 2016/2017, 2017/2018, and 2018/2019 cropping seasons at Baoding, Hebei Province, and the 2016/2017, 2017/2018, and 2018/2019 crop seasons at Zhoukou, Henan Province. Bulked segregant analysis and simple sequence repeat markers were then used to identify the quantitative trait loci (QTLs) for Lr adult-plant resistance in the population. Six QTLs were consequently detected and designated as QLr.hebau-1AL and QLr.hebau-1AS that were presumed to be new and QLr.hebau-1BL, QLr.hebau-3AL, QLr.hebau-4BL, and QLr.hebau-7DS that were identified at similar physical positions as previously reported QTLs. Based on chromosome positions and molecular marker tests, QLr.hebau-1BL and QLr.hebau-7DS share similar flanking markers with Lr46 and Lr34, respectively. Lr46 and Lr34 are race nonspecific adult plant resistance (APR) genes for leaf rust and stripe rust and powdery mildew. QLr.hebau-4BL showed multiple disease resistance to leaf rust, stripe rust, Fusarium head blight, and powdery mildew. The QTL identified in this study, as well as their closely linked markers, may potentially be used in marker-assisted selection in wheat breeding.
摘要：
小麦叶锈病(Lr),是由PucciniatriticinaEriks引起的.(Pt)是影响全球小麦生产的最重要的小麦病害之一。使用抗性小麦品种是控制叶锈病最经济，最环保的方法。意大利小麦品种Libellula在田间研究中表现出对Lr的良好抗性。为了确定Libellula中Lr抗性的遗传基础，来自Libellula/Huixianhong杂交的248F6重组自交系在七个环境中进行了Lr严重程度的表型分析：保定的2014/2015、2016/2017、2017/2018和2018/2019种植季节，河北省和周口的2016/2017、2017/2018和2018/2019作物季节，河南省。然后使用大量的分离分析和简单的序列重复标记来鉴定种群中Lr成年植物抗性的定量性状基因座（QTL）。因此检测到六个QTL，指定为QLr。hebau-1AL和QLr。hebau-1AS假定是新的，QLr。hebau-1BL,QLr。hebau-3AL,QLr。hebau-4BL,和QLr。hebau-7DS在与先前报道的QTL相似的物理位置识别。根据染色体位置和分子标记测试，QLr。hebau-1BL和QLr。hebau-7DS分别与Lr46和Lr34具有相似的侧翼标记。Lr46和Lr34是叶锈病的种族非特异性APR基因，条锈病,和白粉病.同样，QLr。Hebau-4BL对叶锈病表现出多种抗病性，条锈病,镰刀菌枯萎病,和白粉病.本研究中确定的QTL，以及它们紧密相连的标记，有可能用于小麦育种中的标记辅助选择。
