Abstract：
A new electrode management, within the HPAEC-PAD systems, was proposed to measure inulin-type fructans in chicory roots, grown under two lighting periods: 12 h (T-12 h) and 24 h continuous lighting (T-24 h-CL), with the same daily light integral (DLI). The amperometric cell turn-off (PAD-Off) after elution of carbohydrate of interest, allowed the stabilization of the PAD response, avoiding excessive electrode surface oxidation. The enhanced signal stability allowed the application of fucose as internal standard (ISTD) for data normalization, improving the correctness of linear calibration curves and the quantification of fructans in the case study of chicory plants. T-24 h-CL decreased FW and DW of chicory leaves while increasing these parameters in roots. Fructans amount in chicory roots was significantly higher in the T-24-CL photoperiod. The accuracy of prebiotics quantification by PAD-Off emphasized significant differences between light treatments. CL can improve the yield and quality of chicory roots.
摘要：
一种新的电极管理，在HPAEC-PAD系统中，被提议测量菊苣根中的菊粉型果聚糖，在两个光照周期下生长：12小时(T-12小时)和24小时连续光照(T-24小时-CL)，具有相同的日常光积分（DLI）。洗脱目标碳水化合物后的安培细胞关闭（PAD-Off），允许PAD反应的稳定，避免电极表面过度氧化。增强的信号稳定性允许应用岩藻糖作为内标(ISTD)进行数据归一化,以菊苣植物为例，提高线性校准曲线的正确性和果聚糖的定量。T-24h-CL降低了菊苣叶片的FW和DW，同时增加了根中的这些参数。在T-24-CL光周期中，菊苣根中的果聚糖含量明显更高。通过PAD-Off进行益生元定量的准确性强调了光处理之间的显着差异。CL可以提高菊苣根的产量和质量。
