关键词: Cell wall hydrolase Laminin Leptospira LysM M23 Peptidoglycan

Mesh : Humans Laminin / metabolism Lipopolysaccharides / metabolism Peptidoglycan / metabolism Leptospira interrogans / genetics metabolism Hydrolases / metabolism Leptospira / genetics Cell Wall / metabolism Protein Binding

来  源:   DOI:10.1016/j.resmic.2023.104107

Abstract:
Leptospirosis, a global reemerging zoonosis caused by the spirochete Leptospira, has severe human and veterinary implications. Cell wall hydrolase (LIC_10271) with LytM (peptidase M23) and LysM domains are found to be associated with various pathogenic bacteria. These domains regulate effects on extracellular matrix and biofilm components, which promote cell wall remodeling and pathogen dissemination in the host. In this study, we present the cloning, expression, purification, and characterization of LIC_10271. To determine the localization of LIC_10271 within the inner membrane of Leptospira, Triton X-114 subcellular fractionation and immunoblot studies were performed. Furthermore, r-LIC_10271 binds with peptidoglycan, lipopolysaccharide, and laminin in a dose-dependent manner. Analysis of the signal peptide, M23, and LysM domains revealed conservation primarily within the P1 group of Leptospira, which encompasses the most pathogenic species. Moreover, the presence of native-LIC_10271 in the inner membrane and the distribution of M23 and LysM domains across pathogenic strains indicates their potential involvement in the interaction between the host and Leptospira.
摘要:
钩端螺旋体病,由螺旋体钩端螺旋体引起的全球重新出现的人畜共患病,具有严重的人类和兽医影响。发现具有LytM(肽酶M23)和LysM结构域的细胞壁水解酶(LIC_10271)与各种病原菌有关。这些结构域调节对细胞外基质和生物膜成分的影响,促进细胞壁重塑和病原体在宿主中的传播。在这项研究中,我们展示了克隆,表达式,净化,LIC_10271的表征。为了确定LIC_10271在钩端螺旋体内膜内的定位,进行TritonX-114亚细胞分级分离和免疫印迹研究。此外,r-LIC_10271与肽聚糖结合,脂多糖,和层粘连蛋白以剂量依赖的方式。信号肽的分析,M23和LysM结构域主要在钩端螺旋体的P1组内显示出保守性,其中包括最致病的物种。此外,内膜中天然LIC_10271的存在以及致病菌株中M23和LysM结构域的分布表明它们可能参与宿主和钩端螺旋体之间的相互作用。
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