In the present study, urinary catheters were collected from a 50-year-old woman suffering from malignancy, bedridden, and having urinary incontinence. These catheters were processed in laboratory for isolation of bacteria using standard procedures.
Microbiological examination of the urinary catheters by biochemical, physiological, and VITEK 2 compact system revealed bacterial infection caused by Micrococcus lylae, a Gram-positive microorganism belonging to the family Micrococcacea. These Gram-positive bacteria were found to be susceptible to streptomycin, erythromycin, cefotaxime, neomycin, kanamycin, vancomycin, azithromycin, chloramphenicol, and tetracycline. Bacterial species were confirmed using 16s rRNA sequencing.
The sequences were found to have 99% similarity with Micrococcus lylae. This is the first report of isolation of Micrococcus lylae from the urinary catheter.
方法:在本研究中,从一名患有恶性肿瘤的50岁女性身上收集了导尿管,卧床不起,还有尿失禁.在实验室中处理这些导管以使用标准程序分离细菌。
结果:通过生化对导尿管进行微生物学检查,生理,和VITEK2紧凑系统揭示了由碱液微球菌引起的细菌感染,属于微球菌科的革兰氏阳性微生物。发现这些革兰氏阳性细菌对链霉素敏感,红霉素,头孢噻肟,新霉素,卡那霉素,万古霉素,阿奇霉素,氯霉素,还有四环素.使用16srRNA测序确认细菌种类。
结论:发现序列与lylae微球菌有99%的相似性。这是从导尿管中分离出莱莱微球菌的第一份报告。