PDF(Pubmed)
Abstract：
Staphylococcus pseudintermedius is the most common opportunistic pathogen in dogs and methicillin resistance (MRSP) has been identified as an emerging problem in canine pyoderma. Here, we evaluated the antimicrobial resistance (AMR) features and phylogeny of S. pseudintermedius isolated from canine pyoderma cases in Argentina (n = 29) and the United States (n = 29). 62% of isolates showed multi-drug resistance. The AMR genes found: mecA, blaZ, ermB, dfrG, catA, tetM, aac(6\')-aph(2″), in addition to tetK and lnuA (only found in U.S. isolates). Two point mutations were detected: grlA(S80I)-gyrA(S84L), and grlA(D84N)-gyrA(S84L) in one U.S. isolate. A mutation in rpoB (H481N) was found in two isolates from Argentina. SCCmec type III, SCCmec type V, ΨSCCmec57395 were identified in the Argentinian isolates; and SCCmec type III, SCCmec type IVg, SCCmec type V, and SCCmec type VII variant in the U.S. cohort. Sequence type (ST) ST71 belonging to a dominant clone was found in isolates from both countries, and ST45 only in Argentinian isolates. This is the first study to comparatively analyze the population structure of canine pyoderma-associated S. pseudintermedius isolates in Argentina and in the U.S. It is important to maintain surveillance on S. pseudintermedius populations to monitor AMR and gain further understanding of its evolution and dissemination.
摘要：
假中间葡萄球菌是犬中最常见的机会病原体，甲氧西林耐药性（MRSP）已被确定为犬脓皮病的新兴问题。这里,我们评估了从阿根廷(n=29)和美国(n=29)的犬脓皮病病例中分离出的假中间链球菌的抗菌素耐药性(AMR)特征和系统发育。62%的分离株出现多药耐药。发现的AMR基因:mecA,BlaZ,ermB,dfrG,CatA,tetM,aac(6\')-aph(2″)，除了tetK和lnuA（仅在美国分离株中发现）。检测到两个点突变:grlA(S80I)-gyrA(S84L),和grlA(D84N)-gyrA(S84L)在一个美国分离物中。在阿根廷的两个分离株中发现了rpoB（H481N）的突变。SCCmecIII型，SCCmecV型，在阿根廷分离株中鉴定出SCCmec57395；和SCCmecIII型，SCCmec型IVg,SCCmecV型，和SCCmecVII型变异在美国队列中。在这两个国家的分离株中发现了属于优势克隆的序列类型（ST）ST71，和ST45仅在阿根廷分离物中。这是第一项比较分析阿根廷和美国犬脓皮病相关假中介杆菌分离株种群结构的研究重要的是保持对假中介链球菌种群的监测，以监测AMR并进一步了解其演变和传播。
