Abstract：
Tissue-engineered heart valve (TEHV) is a promising alternative to current heart valve substitute. Decellularized porcine aortic heart valves (DAVs) are the most common scaffolds of TEHV. Hard to endothelialization is one of the disadvantages of DAVs. Therefore, we aimed to immobilize endothelial progenitor cell (EPC)-aptamer onto DAVs for accelerating endothelialization. In this study, three groups of scaffolds were constructed: DAVs, aptamer-immobilized DAVs (aptamer-DAVs), and glutaraldehyde crosslinked DAVs (GA-DAVs). The results of flow cytometry revealed that EPC-aptamer was specific to EPCs and was immobilized onto DAVs. Cells adhesion experiments demonstrated that EPCs adhered more tightly onto aptamer-DAVs group than other two groups of scaffolds. And cell proliferation assay indicated that EPCs seeded onto aptamer-DAVs group grew faster than DAVs group and GA-DAVs group. Moreover, dynamic capture experiment in flow conditions revealed that the number of EPCs captured by aptamer-DAVs group was more than other two groups. In conclusion, aptamer-DAVs could specifically promote adhesion and proliferation of EPCs and had ability to capture EPCs in simulated flow condition. This could promote re-endothelialization of scaffolds.
摘要：
组织工程心脏瓣膜（TEHV）是当前心脏瓣膜替代品的有希望的替代品。脱细胞猪主动脉心脏瓣膜(DAVs)是最常见的TEHV支架材料。难以内皮化是DAV的缺点之一。因此,我们的目的是将内皮祖细胞（EPC）适体固定在DAV上，以加速内皮化。在这项研究中,构建了三组支架：DAV，适体固定的DAV（适体-DAV），和戊二醛交联的DAV(GA-DAV)。流式细胞术的结果表明，EPC-适体对EPC具有特异性，并固定在DAV上。细胞粘附实验表明，EPC比其他两组支架更紧密地粘附在适体-DAVs组上。细胞增殖实验表明，接种在适配体-DAVs上的EPC比DAVs组和GA-DAVs组生长更快。此外，流动条件下的动态捕获实验显示，适体-DAVs组捕获的EPC数量多于其他两组。总之,适配体-DAV可以特异性地促进EPCs的粘附和增殖，并具有在模拟流动条件下捕获EPCs的能力。这可以促进支架的再内皮化。
