PDF(Pubmed)
Abstract：
Carbon nanospikes (CNSs) are a new nanomaterial that has enhanced surface roughness and surface oxide concentration, increasing the sensitivity for dopamine detection. However, CNS-modified electrodes (CNSMEs) have not been characterized for other neurochemicals, particularly those with higher oxidation potentials. The purpose of this study was to evaluate CNSMEs for the detection of adenosine, hydrogen peroxide (H2O2), and histamine. The sensitivity increased with CNSs, and signals at CNSMEs were about 3.3 times higher than CFMEs. Normalizing for surface area differences using background currents, CNSMEs show an increased signal of 4.8 times for adenosine, 1.5 times for H2O2, and 2 times for histamine. CNSMEs promoted the formation of secondary products for adenosine and histamine, which enables differentiation from other analytes with similar oxidation potentials. CNSs also selectively enhance the sensitivity for adenosine and histamine compared to H2O2. A scan rate test reveals that adenosine is more adsorption-controlled at CNS electrodes than CFMEs. CNSMEs are antifouling for histamine, with less fouling because the polymers formed after histamine electrooxidation do not adsorb due to an elevated number of edge planes. CNSMEs were useful for detecting each analyte applied in brain slices. Because of the hydrophilic surface compared to CFMEs, CNSMEs also have reduced biofouling when used in tissue. Therefore, CNSMEs are useful for tissue measurements of adenosine, hydrogen peroxide, and histamine with high selectivity and low fouling.
摘要：
碳纳米尖峰（CNSs）是一种新型纳米材料，具有增强的表面粗糙度和表面氧化物浓度，提高多巴胺检测的灵敏度。然而,中枢神经系统修饰电极（CNSME）尚未被表征为其他神经化学物质，特别是那些具有较高的氧化电位。这项研究的目的是评估CNSME用于检测腺苷，过氧化氢(H2O2),和组胺.随着CNSs的增加，灵敏度增加，CNSMES的信号比CFME高约3.3倍。使用背景电流归一化表面积差异，CNSME显示腺苷信号增加4.8倍，H2O2的1.5倍，组胺的2倍。CNSMEs促进了腺苷和组胺次级产品的形成，这使得能够区别于具有相似氧化电位的其他分析物。与H2O2相比,CNSs还选择性地增强对腺苷和组胺的敏感性。扫描速率测试表明，与CFME相比，腺苷在CNS电极上的吸附控制更多。CNSME对组胺有防污作用，污染较少，因为组胺电氧化后形成的聚合物由于边缘平面数量增加而不会吸附。CNSME可用于检测应用于脑切片中的每种分析物。由于与CFME相比具有亲水性表面，CNSME在用于组织时还减少了生物污染。因此,CNSME可用于腺苷的组织测量，过氧化氢,和组胺具有高选择性和低污染。
