Abstract：
To improve bacterial photodynamic inactivation (PDI), this work analyzes the photodynamic effect caused by the combination of photosensitizers (PSs) on two bacterial models and different growth mode. Simultaneous administration of PSs from different families, zinc(II) 2,9,16,23-tetrakis[4-(N-methylpyridyloxy)]phthalocyanine (ZnPPc4+), 5,10,15,20-tetra(4-N,N,N-trimethylammonium phenyl)porphyrin (TMAP4+), meso-tetrakis(9-ethyl-9-methyl-3-carbazoyl)chlorin (TEMCC4+) and 5,10,15,20-tetrakis[4-(3-N,N-dimethylaminopropoxy)phenyl] chlorin (TAPC) was investigated against Staphylococcus aureus and Escherichia coli, in planktonic form, biofilm and growth curve. Various PSs combinations showed greater inactivation compared to when used separately under the same conditions but at twice the concentration. However, differences were found in the effectiveness of the PSs combinations on Gram positive and negative bacteria, as well as in planktonic or biofilm form. Likewise, the combination of three PSs completely stopped E. coli growth under optimal nutritional conditions. PSs combination allows extending the range of light absorption by agents that absorb in different areas of the visible spectrum. Therefore, PDI with combined PSs increases its antimicrobial capacity using agents\' concentrations and light fluences lower than those necessary to cause the same effect as single PS. These advances represent a starting point for future research on the potentiation of PDI promoted by the combined use of PSs.
摘要：
为了改善细菌光动力灭活（PDI），这项工作分析了光敏剂（PSs）对两种细菌模型和不同生长方式的组合引起的光动力效应。同时给予来自不同家庭的PS，锌(II)2,9,16,23-四[4-(N-甲基吡啶基氧基)]酞菁(ZnPPc4+),5,10,15,20-tetra(4-N,N,N-三甲基铵苯基)卟啉(TMAP4+)，meso-四(9-乙基-9-甲基-3-咔唑基)氯(TEMCC4+)和5,10,15,20-四[4-(3-N,研究了N-二甲基氨基丙氧基）苯基]三氯[TAPC]对金黄色葡萄球菌和大肠杆菌的作用，以浮游形式，生物膜和生长曲线。与在相同条件下但以两倍的浓度单独使用时相比，各种PS组合显示出更大的失活。然而,PSs组合对革兰氏阳性和阴性细菌的有效性存在差异，以及浮游或生物膜形式。同样，三种PS的组合在最佳营养条件下完全停止了大肠杆菌的生长。PS组合允许通过在可见光谱的不同区域中吸收的试剂来扩展光吸收的范围。因此,PDI与组合PS使用的药剂浓度和光通量低于引起与单一PS相同的效果所必需的浓度和光通量，可提高其抗微生物能力。这些进展代表了通过PS的组合使用促进PDI增强的未来研究的起点。
