This review paper explores the range of analytical approaches for monitoring metabolic pathways, as well as physicochemical modeling techniques, with a focus on glutamine metabolism. We discuss the advantages and disadvantages of each method and explore the potential of label-free Raman microspectroscopy, in conjunction with kinetic modeling, to enable real-time and in situ monitoring of the cellular kinetics of the glutamine metabolic pathway.
Given its important role in cell metabolism, the ability to monitor and model the glutamine metabolic pathways are highlighted. Novel, label free approaches have the potential to revolutionise metabolic biosensing, laying the foundation for a new paradigm in metabolomics research and addressing the challenges in monitoring metabolic pathways in living cells.
目的:这篇综述论文探讨了监测代谢途径的分析方法的范围,以及物理化学建模技术,专注于谷氨酰胺代谢。我们讨论了每种方法的优缺点,并探索了无标记拉曼显微光谱的潜力,结合动力学建模,实时和原位监测谷氨酰胺代谢途径的细胞动力学。
■鉴于其在细胞代谢中的重要作用,监测和模拟谷氨酰胺代谢途径的能力被强调.小说,无标签方法有可能彻底改变代谢生物传感,为代谢组学研究的新范式奠定基础,并解决监测活细胞代谢途径的挑战。