PDF(Pubmed)
Abstract：
BACKGROUND: Milk fat globule-epidermal growth factor 8 (MFG-E8) is expressed in the endometrial epithelium and its expression increases during the implantation process. Due to this knowledge, we aimed to investigate the maternal serum MFG-E8 levels on both healthy pregnant women in the first trimester and pregnant women complicated with missed abortion and threatened abortion in the first trimester.
METHODS: This prospective, cross-sectional study was conducted in a tertiary referral hospital, department of obstetrics between July 2020 and February 2021 after ethical committee approval. The study population was consisted of 30 healthy pregnant women (HP) in the first trimester, 30 pregnant women suffering from threatened abortion (TA) in the first trimester and 30 pregnant women suffering from missed abortion (MA) in the first trimester. Maternal serum MFG-E8 levels were analyzed with enzyme linked immunosorbent assay. Delivery and neonatal outcomes of the study population was evaluated. The continuous variables were compared among three groups with variance analysis with post hoc tests. The categorical variables were compared with chi-square and Fisher\'s exact tests where applicable.
RESULTS: The mean age of the study population was 29.36 ± 5.31 years. There was no significant difference among three groups for parameters of age, body mass index, parity number, and gestational week. Despite being within normal ranges, the mean neutrophil and international normalized ratio values of the three groups showed statistically significant difference (p < 0.05). The mean maternal serum MFG-E8 levels of MA, TA, and HP groups were 270 ± 152.3, 414.7 ± 236.7, and 474 ± 222.5 ng/mL, respectively (p = 0.001). It was found that mean of MFG-E8 of the MA group was statistically significantly lower than those of the other two groups (p < 0.05).
CONCLUSIONS: Although maternal serum MFG-E8 level seems to be a parameter that differ between live and nonlive pregnancies, studies with large number of cases are needed to discuss our results and to determine a cut-off value for prediction.
METHODS: This prospective, cross-sectional study was conducted in a tertiary referral hospital, department of obstetrics between July 2020 and February 2021 after ethical committee approval. The study population was consisted of 30 healthy pregnant women (HP) in the first trimester, 30 pregnant women suffering from threatened abortion (TA) in the first trimester and 30 pregnant women suffering from missed abortion (MA) in the first trimester. Maternal serum MFG-E8 levels were analyzed with enzyme linked immunosorbent assay. Delivery and neonatal outcomes of the study population was evaluated. The continuous variables were compared among three groups with variance analysis with post hoc tests. The categorical variables were compared with chi-square and Fisher\'s exact tests where applicable.
RESULTS: The mean age of the study population was 29.36 ± 5.31 years. There was no significant difference among three groups for parameters of age, body mass index, parity number, and gestational week. Despite being within normal ranges, the mean neutrophil and international normalized ratio values of the three groups showed statistically significant difference (p < 0.05). The mean maternal serum MFG-E8 levels of MA, TA, and HP groups were 270 ± 152.3, 414.7 ± 236.7, and 474 ± 222.5 ng/mL, respectively (p = 0.001). It was found that mean of MFG-E8 of the MA group was statistically significantly lower than those of the other two groups (p < 0.05).
CONCLUSIONS: Although maternal serum MFG-E8 level seems to be a parameter that differ between live and nonlive pregnancies, studies with large number of cases are needed to discuss our results and to determine a cut-off value for prediction.
摘要：
背景：乳脂球-表皮生长因子8（MFG-E8）在子宫内膜上皮中表达，其表达在植入过程中增加。由于这些知识,我们旨在调查孕早期健康孕妇和孕早期合并稽留流产和先兆流产的孕妇的母体血清MFG-E8水平。
方法：这种前瞻性，横断面研究是在三级转诊医院进行的，在道德委员会批准后，产科在2020年7月至2021年2月之间。研究人群由30名健康孕妇（HP）在妊娠早期，30名孕妇在妊娠早期患有先兆流产（TA），30名孕妇在妊娠早期患有流产（MA）。采用酶联免疫吸附试验分析孕妇血清MFG-E8水平。评估研究人群的分娩和新生儿结局。用方差分析和事后检验比较三组之间的连续变量。在适用的情况下，将分类变量与卡方检验和Fisher精确检验进行比较。
结果：研究人群的平均年龄为29.36±5.31岁。三组之间的年龄参数没有显着差异，身体质量指数,奇偶校验号,和孕周。尽管在正常范围内，3组中性粒细胞平均值和国际标准化比值比较,差异有统计学意义(p<0.05)。平均母体血清MFG-E8水平的MA，TA,HP组分别为270±152.3、414.7±236.7和474±222.5ng/mL，分别(p=0.001)。发现MA组的MFG-E8的平均值在统计学上显着低于其他两组（p<0.05）。
结论：尽管孕妇血清MFG-E8水平似乎是一个在活体妊娠和非活体妊娠中不同的参数，需要大量病例的研究来讨论我们的结果并确定预测的临界值.
方法：这种前瞻性，横断面研究是在三级转诊医院进行的，在道德委员会批准后，产科在2020年7月至2021年2月之间。研究人群由30名健康孕妇（HP）在妊娠早期，30名孕妇在妊娠早期患有先兆流产（TA），30名孕妇在妊娠早期患有流产（MA）。采用酶联免疫吸附试验分析孕妇血清MFG-E8水平。评估研究人群的分娩和新生儿结局。用方差分析和事后检验比较三组之间的连续变量。在适用的情况下，将分类变量与卡方检验和Fisher精确检验进行比较。
结果：研究人群的平均年龄为29.36±5.31岁。三组之间的年龄参数没有显着差异，身体质量指数,奇偶校验号,和孕周。尽管在正常范围内，3组中性粒细胞平均值和国际标准化比值比较,差异有统计学意义(p<0.05)。平均母体血清MFG-E8水平的MA，TA,HP组分别为270±152.3、414.7±236.7和474±222.5ng/mL，分别(p=0.001)。发现MA组的MFG-E8的平均值在统计学上显着低于其他两组（p<0.05）。
结论：尽管孕妇血清MFG-E8水平似乎是一个在活体妊娠和非活体妊娠中不同的参数，需要大量病例的研究来讨论我们的结果并确定预测的临界值.
