Abstract：
After an acute intake of 300 g of mango purée by 10 subjects, 0 and 24 h urine and plasma samples were analyzed by high-performance liquid chromatography-high-resolution mass spectrometry. The method was first validated for 44 reference polyphenols in terms of linearity, specificity, limits of detection and quantification, intra-day and inter-day precision, recovery, and matrix effects in two biological matrices. After method validation, a total of 94 microbial-derived phenolic catabolites, including 15 cinnamic acids, 3 phenylhydracrylic acids, 14 phenylpropanoic acids, 12 phenylacetic acids, 28 benzoic acids, 2 mandelic acids, 15 hydroxybenzenes, and 5 hippuric acid derivatives, were identified or tentatively identified in urine and/or plasma. These results establish the value of the UHPLC-HRMS protocol and the use of authentic standards to obtain a detailed and accurate picture of mango polyphenol metabolites, together with their phase II conjugated metabolites, in human bioavailability studies.
摘要：
在10名受试者急性摄入300克芒果泥之后，通过高效液相色谱-高分辨率质谱分析0和24h尿液和血浆样品。该方法首次在线性方面对44种参考多酚进行了验证，特异性，检测和定量的极限，日内和日间精度，recovery,和两种生物基质中的基质效应。方法验证后，总共94种微生物衍生的酚类分解代谢物，包括15种肉桂酸,3苯基羟基丙烯酸，14苯基丙酸，12苯乙酸，28苯甲酸，2扁桃酸，15羟基苯，和5种马尿酸衍生物，在尿液和/或血浆中被鉴定或初步鉴定。这些结果建立了UHPLC-HRMS协议的价值，并使用真实的标准来获得芒果多酚代谢物的详细准确图片，以及它们的II期结合代谢物，在人体生物利用度研究中。
