PDF(Pubmed)
Abstract：
Respiratory disease is a significant economic issue in pig farming, with a complex aetiology that includes swine influenza A viruses (swIAV), which are common in European domestic pig populations. The most recent human influenza pandemic in 2009 showed swIAV\'s zoonotic potential. Monitoring pathogens and disease control are critical from a preventive standpoint, and are based on quick, sensitive, and specific diagnostic assays capable of detecting and distinguishing currently circulating swIAV in clinical samples. For passive surveillance, a set of multiplex quantitative reverse transcription real-time PCRs (mRT-qPCR) and MinION-directed sequencing was updated and deployed. Several lineages and genotypes of swIAV were shown to be dynamically developing, including novel reassortants between human pandemic H1N1 and the avian-derived H1 lineage of swIAV. Despite this, nearly 70% (842/1216) of individual samples from pigs with respiratory symptoms were swIAV-negative, hinting to different aetiologies. The complex and synergistic interactions of swIAV infections with other viral and bacterial infectious agents contribute to the aggravation of pig respiratory diseases. Using a newly developed mRT-qPCR for the combined detection of swIAV and the recently described porcine respirovirus 1 (PRV1) and swine orthopneumovirus (SOV) widespread co-circulation of PRV1 (19.6%, 238/1216 samples) and SOV (14.2%, 173/1216 samples) was evident. Because of the high incidence of PRV1 and SOV infections in pigs with respiratory disease, these viruses may emerge as new allies in the porcine respiratory disease syndrome.
摘要：
呼吸道疾病是养猪的重大经济问题,具有复杂的病因，包括猪甲型流感病毒（swIAV），这在欧洲家猪种群中很常见。2009年最近的人类流感大流行显示了swIAV的人畜共患潜力。从预防的角度来看，监测病原体和疾病控制至关重要，基于快速，敏感,以及能够检测和区分临床样品中当前循环的swIAV的特异性诊断测定。对于被动监视，更新并部署了一组多重定量逆转录实时PCR(mRT-qPCR)和MinION定向测序.SwIAV的几种谱系和基因型被证明是动态发展的，包括人类大流行H1N1和SwIAV的禽源H1谱系之间的新重排。尽管如此,近70%(842/1216)来自有呼吸道症状的猪的个体样本为SwIAV阴性,暗示不同的病因。swIAV感染与其他病毒和细菌感染因子的复杂和协同相互作用有助于猪呼吸道疾病的加重。使用新开发的mRT-qPCR联合检测swIAV和最近描述的猪呼吸道病毒1（PRV1）和猪正肺病毒（SOV）广泛的PRV1共同循环（19.6％，238/1216个样品）和SOV（14.2%，173/1216个样品）很明显。由于呼吸道疾病猪PRV1和SOV感染的发病率较高，这些病毒可能成为猪呼吸道疾病综合征的新盟友。
