PDF(Pubmed)
Abstract：
Using natural dyes in dyeing industries becomes an alternative to synthetic dyes, which are known to contain harmful chemicals that can pose risks to the environment and human health. This study involves the extraction of yellow dye from Cassia alata flower petals, optimization of the extraction process using an ultrasonic bath (40 KHz and an input power of 500), ultrasonic probe (390 W, 455 W, 520 W, 585 W, and 650 W), and conventional heating (heating mantle with 30 °C, 40 °C, 50 °C, 60 °C, and 70 °C), characterization of the dye, as well as dyeing (cotton, silk, and leather) without using a mordant. The extracted yellow dye was further evaluated to determine its antibacterial activity against skin bacteria. Dye extraction optimization using UV-Visible spectrophotometric analysis revealed that the maximum yellow color in methanol extract (287 and 479 nm) was obtained at 50 °C for 45 min using ultrasonic water bath extraction, followed by the ultrasonic probe and direct heating. Based on the FTIR spectra, it is evident that OH is present at approximately 3300 cm-1, while CH stretches at around 2900 cm-1. A characteristic peak at 1608 cm-1 bears a striking similarity to anthraquinonoid-based compounds. Also, using the ultrasonic water bath dyeing technique at 50 °C for 45 min, the yellow color of cotton, silk, and leather was dyed optimally. Due to effective color removal after two washings with boiling soap liquid, the dyed cotton and silk fabric displayed good washing and rubbing fastness. Regarding antibacterial activity, the dye was highly active against all pathogens after extraction in methanol. The maximum inhibition was observed against Pseudomonas sp. with a MIC value of 1.56 mg/ml.
摘要：
在染色行业中使用天然染料成为合成染料的替代品，已知含有有害化学物质，可能对环境和人类健康构成威胁。本研究涉及从决明子花瓣中提取黄色染料，使用超声波浴（40KHz，输入功率为500）优化提取过程，超声波探头(390W,455W,520W,585瓦,和650瓦），和常规加热(30°C的加热罩，40°C,50°C,60°C,和70°C），染料的表征，以及染色(棉，丝绸,和皮革）不使用媒染剂。进一步评价提取的黄色染料以确定其对皮肤细菌的抗菌活性。使用UV-可见光分光光度分析进行染料提取优化，结果表明，使用超声波水浴提取在50°C下45分钟，甲醇提取物（287和479nm）中获得最大的黄色，其次是超声波探头和直接加热。基于FTIR光谱,显然,OH存在于约3300cm-1处,而CH在约2900cm-1处延伸。1608cm-1的特征峰与基于蒽醌的化合物具有惊人的相似性。此外，使用超声波水浴染色技术在50°C下持续45分钟，棉花的黄色，丝绸,皮革染色效果最佳。由于用沸腾的肥皂液洗涤两次后可有效去除颜色，染色棉和丝织物显示出良好的洗涤和摩擦牢度。关于抗菌活性，在甲醇中提取后，染料对所有病原体都具有很高的活性。观察到对假单胞菌的最大抑制作用。MIC值为1.56mg/ml。
