PDF(Pubmed)
Abstract：
BACKGROUND: Microsatellite markers were developed for distylous Linum suffruticosum and tested in the monomorphic sister species Linum tenuifolium. These species are perennial herbs endemic to the western and northwestern Mediterranean, respectively, with a partially overlapping distribution area.
RESULTS: We developed 12 microsatellite markers for L. suffruticosum using next generation sequencing, and assessed their polymorphism and genetic diversity in 152 individuals from seven natural populations. The markers displayed high polymorphism, with two to 16 alleles per locus and population, and average observed and expected heterozygosities of 0.833 and 0.692, respectively. All loci amplified successfully in the sister species L. tenuifolium, and 150 individuals from seven populations were also screened. The polymorphism exhibited was high, with two to ten alleles per locus and population, and average observed and expected heterozygosities of 0.77 and 0.62, respectively.
CONCLUSIONS: The microsatellite markers identified in L. suffruticosum and tested in L. tenuifolium are a powerful tool to facilitate future investigations of the population genetics, mating patterns and hybridization between both Linum species in their contact zone.
RESULTS: We developed 12 microsatellite markers for L. suffruticosum using next generation sequencing, and assessed their polymorphism and genetic diversity in 152 individuals from seven natural populations. The markers displayed high polymorphism, with two to 16 alleles per locus and population, and average observed and expected heterozygosities of 0.833 and 0.692, respectively. All loci amplified successfully in the sister species L. tenuifolium, and 150 individuals from seven populations were also screened. The polymorphism exhibited was high, with two to ten alleles per locus and population, and average observed and expected heterozygosities of 0.77 and 0.62, respectively.
CONCLUSIONS: The microsatellite markers identified in L. suffruticosum and tested in L. tenuifolium are a powerful tool to facilitate future investigations of the population genetics, mating patterns and hybridization between both Linum species in their contact zone.
摘要：
背景：微卫星标记是为双叶亚麻子开发的，并在单态姐妹物种亚麻子中进行了测试。这些物种是地中海西部和西北部特有的多年生草本植物，分别,具有部分重叠的分布区域。
结果：我们使用下一代测序技术开发了12个微卫星标记，并评估了来自七个自然种群的152个个体的多态性和遗传多样性。标记显示高度多态性，每个基因座和种群有2到16个等位基因，平均观察和预期杂合度分别为0.833和0.692。在姊妹种L.tenuifolium中成功扩增了所有基因座，并对来自7个群体的150名个体进行了筛选。表现出的多态性很高，每个基因座和种群有两到十个等位基因，平均观察和预期杂合度分别为0.77和0.62。
结论：在甘草中鉴定并在甘草中测试的微卫星标记是促进未来种群遗传学研究的有力工具，两个Linum物种在其接触区中的交配模式和杂交。
结果：我们使用下一代测序技术开发了12个微卫星标记，并评估了来自七个自然种群的152个个体的多态性和遗传多样性。标记显示高度多态性，每个基因座和种群有2到16个等位基因，平均观察和预期杂合度分别为0.833和0.692。在姊妹种L.tenuifolium中成功扩增了所有基因座，并对来自7个群体的150名个体进行了筛选。表现出的多态性很高，每个基因座和种群有两到十个等位基因，平均观察和预期杂合度分别为0.77和0.62。
结论：在甘草中鉴定并在甘草中测试的微卫星标记是促进未来种群遗传学研究的有力工具，两个Linum物种在其接触区中的交配模式和杂交。
