Abstract：
Fasciola hepatica, the liver trematode, infects ruminants and causes economic loss. Because parasites are developing resistance to commercial drugs, the negative effects of parasitism are increasing. In this study, we aimed to evaluate the efficacy of cumin (Cuminum cyminum) essential oil against F. hepatica eggs and adults. The eggs were incubated with eight concentrations of the essential oil (0.031125-4.15 mg/mL), and viable eggs were counted after 14 days and classified as embryonated or non-embryonated. Adult flukes were incubated in Roswell Park Memorial Institute medium to ensure their viability and then incubated in essential oil. They were observed for 24 h after treatment. The adults were assessed with the two lowest effective oil concentrations used in the ovicidal test. Three controls were used for both tests: nitroxynil, a negative control, and Tween®80. After incubation in oil, the adult specimens were processed for histological analysis and stained with hematoxylin-eosin. In addition, the oil was tested for cytotoxicity using Madin-Darby bovine kidney cells to assess any possible effect on them. The oil was effective in ovicidal and adulticidal inhibition of the trematode, with statistically significant results. All concentrations assessed in the ovicidal test were 100% effective. The adult test was effective within 15 h and inactivated all the specimens at the highest concentration evaluated (0.06225 mg/mL). Histological analysis showed that cumin essential oil resulted in marked areas of vacuolization. The spines showed no structural changes but were surrounded by microvesicles. These findings indicated that cumin oil could be a potential compound in the control of fasciolosis.
摘要：
肝片吸虫,肝脏吸虫，感染反刍动物并造成经济损失。因为寄生虫正在对商业药物产生抗药性，寄生的负面影响越来越大。在这项研究中,我们的目的是评估孜然（CuminumCuminumCuminumCuminumCuminumCuminumCuminum）精油对肝肝菌卵和成虫的疗效。将鸡蛋与八种浓度的精油（0.031125-4.15mg/mL）一起孵育，和活卵在14天后计数并分类为含胚或非含胚。将成年吸虫在罗斯威尔公园纪念研究所的培养基中孵育，以确保其生存能力，然后在精油中孵育。治疗后24h观察。用杀卵试验中使用的两种最低有效油浓度对成年人进行评估。两项测试都使用了三个对照：硝基苯腈，阴性对照，和Tween®80。在油中孵育后，对成人标本进行组织学分析,并用苏木精-伊红染色.此外,使用Madin-Darby牛肾细胞测试油的细胞毒性，以评估对它们的任何可能的影响。该油可有效抑制吸虫的杀卵和杀成虫，具有统计学意义的结果。在杀卵试验中评估的所有浓度均为100%有效。成人测试在15小时内有效，并在评估的最高浓度（0.06225mg/mL）下灭活所有样本。组织学分析表明，孜然精油导致明显的空泡化区域。棘没有结构变化，但被微囊泡包围。这些发现表明孜然油可能是控制筋膜病的潜在化合物。
