Abstract：
The flatworm planarian, Schmidtea mediterranea (Smed) is a master at regenerating and rebuilding whole animals from fragments. A full understanding of Smed\'s regenerative capabilities requires a high-resolution characterization of organs, tissues, and the adult stem cells necessary for regeneration in their native environment. Here, we describe a serial block face scanning electron microscopy (SBF-SEM) protocol, optimized for Smed specifically, for visualizing the ultrastructure of membranes and condensed chromosomes in this model organism.
摘要：
扁平虫涡虫，Schmidteamediterranea（Smed）是从碎片再生和重建整个动物的大师。充分了解Smed的再生能力需要对器官进行高分辨率表征，组织，以及在天然环境中再生所必需的成体干细胞。这里,我们描述了一种串行块面扫描电子显微镜(SBF-SEM)协议，特别针对Smed进行了优化，用于可视化该模型生物中膜和浓缩染色体的超微结构。
