Abstract：
Affinity purification-Mass spectroscopy (AP-MS) is a biochemical technique to identify the novel protein-protein interaction that occurs in the most relevant physiological conditions, whereas co-immunoprecipitation (Co-IP) is used to study the interaction between two known protein partners that are expressed in the native physiological conditions. Both AP-MS and Co-IP techniques are based on the ability of the interacting partners to pull-down with protein of interest. In this chapter, we have explained the AP-MS and Co-IP methods to study protein-protein interactions in the plant cells.
摘要：
亲和纯化-质谱（AP-MS）是一种生化技术，用于鉴定在最相关的生理条件下发生的新型蛋白质-蛋白质相互作用。而免疫共沉淀（Co-IP）用于研究在天然生理条件下表达的两种已知蛋白质伴侣之间的相互作用。AP-MS和Co-IP技术都是基于相互作用的配偶体拉下感兴趣的蛋白质的能力。在这一章中,我们已经解释了AP-MS和Co-IP方法来研究植物细胞中的蛋白质-蛋白质相互作用。
