PDF(Pubmed)
Abstract：
Red clover is a raw material of interest primarily due to its isoflavone content. However, other groups of compounds may affect the pleiotropic biological effects of this raw material. It is used to alleviate menopausal symptoms, but the fact that there are many varieties of this plant that can be grown makes it necessary to compare the biological activity and phytochemical composition of this plant. Also of interest are the differences between the leaves and flowers of the plant. The aim of this study was to evaluate the properties of the leaves and flowers of six clover varieties-\'Tenia\', \'Atlantis\', \'Milena\', \'Magellan\', \'Lemmon\' and \'Lucrum\'-with respect to their ability to inhibit α-glucosidase, lipase, collagenase and antioxidant activity. Therefore, the contents of polyphenols and the four main isoflavones-genistein, daidzein, biochanin and formononetin-were assessed. The study was complemented by testing for permeability through a model membrane system (PAMPA). Principal component analysis (PCA) identified a relationship between activity and the content of active compounds. It was concluded that antioxidant activity, inhibition of glucosidase, collagenase and lipase are not correlated with isoflavone content. A higher content of total polyphenols (TPC) was determined in the flowers of red clover while a higher content of isoflavones was determined in the leaves of almost every variety. The exception is the \'Lemmon\' variety, characterized by high isoflavone content and high activity in the tests conducted.
摘要：
红三叶草是一种令人感兴趣的原料，主要是由于其异黄酮含量。然而,其他化合物可能会影响该原料的多效性生物学效应。它用于缓解更年期症状，但是这种植物有许多品种可以种植的事实使得有必要比较这种植物的生物活性和植物化学成分。还感兴趣的是植物的叶和花之间的差异。这项研究的目的是评估六个三叶草品种的叶子和花的特性-“Tenia”，\'亚特兰蒂斯\',\'Milena\',\'麦哲伦\',\'Lemmon\'和\'Lucrum\'-关于它们抑制α-葡萄糖苷酶的能力，脂肪酶,胶原酶和抗氧化活性。因此,多酚和四种主要异黄酮-金雀异黄素的含量，Daidzein,对Biochanin和Formononetin进行了评估。该研究通过测试通过模型膜系统(PAMPA)的渗透性来补充。主成分分析（PCA）确定了活性与活性化合物含量之间的关系。结论是抗氧化活性，抑制葡萄糖苷酶，胶原酶和脂肪酶与异黄酮含量无关。在红三叶草的花朵中确定了较高的总多酚（TPC）含量，而在几乎每个品种的叶子中都确定了较高的异黄酮含量。唯一的例外是\'Lemmon\'品种，在进行的测试中，其特点是异黄酮含量高，活性高。
