PDF(Pubmed)
Abstract：
Temporal lobe epilepsy (TLE) is the most common form of epilepsy in adults. Tissue reorganization at the site of the epileptogenic focus is accompanied by changes in the expression patterns of protein molecules. The study of mRNA and its corresponding proteins is crucial for understanding the pathogenesis of the disease. Protein expression profiles do not always directly correlate with the levels of their transcripts; therefore, it is protein profiling that is no less important for understanding the molecular mechanisms and biological processes of TLE. The study and annotation of proteins that are statistically significantly different in patients with TLE is an approach to search for biomarkers of this disease, various stages of its development, as well as a method for searching for specific targets for the development of a further therapeutic strategy. When writing a systematic review, the following aggregators of scientific journals were used: MDPI, PubMed, ScienceDirect, Springer, and Web of Science. Scientific articles were searched using the following keywords: \"proteomic\", \"mass-spectrometry\", \"protein expression\", \"temporal lobe epilepsy\", and \"biomarkers\". Publications from 2003 to the present have been analyzed. Studies of brain tissues, experimental models of epilepsy, as well as biological fluids, were analyzed. For each of the groups, aberrantly expressed proteins found in various studies were isolated. Most of the studies omitted important characteristics of the studied patients, such as: duration of illness, type and response to therapy, gender, etc. Proteins that overlap across different tissue types and different studies have been highlighted: DPYSL, SYT1, STMN1, APOE, NME1, and others. The most common biological processes for them were the positive regulation of neurofibrillary tangle assembly, the regulation of amyloid fibril formation, lipoprotein catabolic process, the positive regulation of vesicle fusion, the positive regulation of oxidative stress-induced intrinsic apoptotic signaling pathway, removal of superoxide radicals, axon extension, and the regulation of actin filament depolymerization. MS-based proteomic profiling for a relevant study must accept a number of limitations, the most important of which is the need to compare different types of neurological and, in particular, epileptic disorders. Such a criterion could increase the specificity of the search work and, in the future, lead to the discovery of biomarkers for a particular disease.
摘要：
颞叶癫痫（TLE）是成人最常见的癫痫形式。癫痫灶部位的组织重组伴随着蛋白质分子表达模式的变化。mRNA及其相应蛋白质的研究对于理解疾病的发病机理至关重要。蛋白质表达谱并不总是与其转录物的水平直接相关；因此，蛋白质谱分析对于理解TLE的分子机制和生物学过程同样重要。对TLE患者中具有统计学上显著差异的蛋白质的研究和注释是寻找该疾病生物标志物的一种方法，其发展的各个阶段，以及寻找特定靶标以开发进一步的治疗策略的方法。在撰写系统综述时，使用了以下科学期刊的聚合器：MDPI，PubMed,ScienceDirect,Springer,和WebofScience。使用以下关键字搜索科学文章：“蛋白质组学”，“质谱”，“蛋白质表达”，“颞叶癫痫”，和“生物标志物”。对2003年至今的出版物进行了分析。脑组织的研究，癫痫的实验模型，以及生物流体，进行了分析。对于每个小组，分离了在各种研究中发现的异常表达的蛋白质。大多数研究都忽略了被研究患者的重要特征，例如：疾病的持续时间，类型和对治疗的反应，性别,等。强调了在不同组织类型和不同研究中重叠的蛋白质：DPYSL，SYT1,STMN1,APOE,NME1等。它们最常见的生物过程是神经原纤维缠结组装的正向调节，淀粉样蛋白原纤维形成的调节，脂蛋白分解代谢过程，囊泡融合的正向调节，氧化应激诱导的内源性凋亡信号通路的正向调节，去除超氧自由基，轴突延伸,以及肌动蛋白丝解聚的调节。相关研究的基于MS的蛋白质组学分析必须接受许多限制，其中最重要的是需要比较不同类型的神经系统，特别是,癫痫症.这样的标准可以增加搜索工作的特殊性，在未来,导致发现特定疾病的生物标志物。
