Amplification of Extracted DNA and Direct Amplification with the PowerPlex® Y23 System.

用 PowerPlex ® Y23 系统扩增提取的 DNA 和直接扩增。

• 发表时间: 2023
• 来源期刊: Methods Mol Biol
• DOI: 10.1007/978-1-0716-3295-6_14
• PMID: 37439985
• 文章类型: Journal Article
• 作者列表: Thompson JM

关键词: DNA typing; Direct amplification; Polymerase chain reaction (PCR); PowerPlex® Y23; Short tandem repeat (STR); Y-STR

Mesh: Humans; Microsatellite Repeats; Chromosomes, Human, Y; DNA / genetics; DNA Fingerprinting; Databases, Factual; Haplotypes; Genetics, Population

Abstract

The PowerPlex® Y23 System offered by Promega Corporation contains 23 Y-STR loci (DYS19, DYS385a/b, DYS389I/II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS438, DYS439, DYS448, DYS456, DYS458, DYS481, DYS533, DYS549, DYS570, DYS576, DYS635, DYS643, and Y-GATA-H4). The PowerPlex® Y23 System is designed to amplify DNA from purified extracts as well as direct amplification from substrates used to collect database samples (e.g., swabs and storage cards). Protocols are provided for full-volume reactions for DNA extracts, as well as half-volume reactions for direct amplifications from different substrates.

摘要

PromegaCorporation提供的PowerPlex®Y23系统包含23个Y-STR基因座(DYS19、DYS385a/b、DYS389I/II,DYS390、DYS391、DYS392、DYS393、DYS437、DYS438、DYS439、DYS448、DYS456、DYS458、DYS481、DYS533、DYS549、DYS570、DYS576、DYS635、DYS643和Y-GPowerPlex®Y23系统旨在从纯化的提取物中扩增DNA，以及从用于收集数据库样品的底物中直接扩增(例如，拭子和存储卡)。提供了用于DNA提取物的全体积反应的方案，以及来自不同底物的直接扩增的半体积反应。