Abstract：
Cell viability largely depends on the surveillance of mRNA export and translation. Upon pre-mRNA processing and nuclear quality control, mature mRNAs are exported into the cytoplasm via Mex67-Mtr2 attachment. At the cytoplasmic site of the nuclear pore complex, the export receptor is displaced by the action of the DEAD-box RNA helicase Dbp5. Subsequent quality control of the open reading frame requires translation. Our studies suggest an involvement of Dbp5 in cytoplasmic no-go-and non-stop decay. Most importantly, we have also identified a key function for Dbp5 in translation termination, which identifies this helicase as a master regulator of mRNA expression.
摘要：
细胞活力很大程度上取决于mRNA输出和翻译的监测。经过mRNA前加工和核质量控制，成熟的mRNA通过Mex67-Mtr2连接输出到细胞质中。在核孔复合体的细胞质位点，输出受体被DEAD-boxRNA解旋酶Dbp5的作用所取代。开放阅读框的后续质量控制需要翻译。我们的研究表明，Dbp5参与了细胞质的不停止和不停止衰变。最重要的是,我们还确定了翻译终止中Dbp5的关键函数，这表明该解旋酶是mRNA表达的主要调节因子。
