PDF(Pubmed)
Abstract：
We systematically investigate functional and molecular measures of stemness in patients with acute myeloid leukemia (AML) using a cohort of 121 individuals. We confirm that the presence of leukemic stem cells (LSCs) detected through in vivo xenograft transplantation is associated with poor survival. However, the measurement of leukemic progenitor cells (LPCs) through in vitro colony-forming assays provides an even stronger predictor of overall and event-free survival. LPCs not only capture patient-specific mutations but also retain serial re-plating ability, demonstrating their biological relevance. Notably, LPC content represents an independent prognostic factor in multivariate analyses including clinical guidelines of risk stratification. Our findings suggest that LPCs provide a robust functional measure of AML, enabling quantitative and rapid assessment of a wide range of patients. This highlights the potential of LPCs as a valuable prognostic factor in AML management.
摘要：
我们使用121例患者的队列，系统地研究了急性髓细胞性白血病（AML）患者的功能和分子指标。我们证实，通过体内异种移植检测到的白血病干细胞（LSCs）的存在与低存活率相关。然而,通过体外集落形成试验对白血病祖细胞(LPCs)的检测提供了更强的总体生存和无事件生存预测指标.LPC不仅捕获患者特异性突变，而且还保留了连续的重新接种能力，证明了它们的生物学相关性。值得注意的是,LPC含量在多变量分析中代表独立的预后因素，包括风险分层的临床指南。我们的研究结果表明，LPC提供了一个强大的AML功能指标，能够定量和快速评估广泛的患者。这凸显了LPC作为AML管理中一个有价值的预后因素的潜力。
