PDF(Pubmed)
Abstract：
Cryptorchidism is a congenital abnormality resulting in increased rates of infertility and testicular cancer. We used cryptorchidism model mice that presented with the translocation of the left testis from the scrotum to the abdominal cavity. Mice underwent the surgical procedure of the left testis at day 0 and were sacrificed at days 3, 5, 7, 14, 21, and 28 post-operatively. The weight of the left cryptorchid testis decreased significantly at days 21 and 28. The morphological changes were observed after 5 days and showed detached spermatogenic cells and abnormal formation of acrosome at day 5, multinucleated giant cells at day 7, and atrophy of seminiferous tubules at days 21 and 28. The high abdominal temperature disrupted the normal expression of cell adhesion molecule-1, Nectin-2, and Nectin-3 which are essential for spermatogenesis. In addition, the pattern and alignment of acetylated tubulin in cryptorchid testes were also changed at days 5, 7, 14, 21, and 28. Ultrastructure of cryptorchid testes revealed giant cells that had been formed by spermatogonia, spermatocytes, and round and elongating spermatids. The study\'s findings reveal that cryptorchidism\'s duration is linked to abnormal changes in the testis, impacting protein marker expression in spermatogenic and Sertoli cells. These changes stem from the induction of high abdominal temperature.
摘要：
隐睾是一种先天性异常，导致不育和睾丸癌的发病率增加。我们使用隐睾模型小鼠，其表现为左睾丸从阴囊到腹腔的易位。小鼠在第0天进行左睾丸的外科手术,并在术后第3、5、7、14、21和28天处死。左侧隐睾睾丸的重量在第21和28天明显下降。5天后观察形态变化，第5天生精细胞脱落，顶体异常形成，第7天多核巨细胞，第21和28天生精小管萎缩。腹部高温破坏了精子发生所必需的细胞粘附分子1，Nectin-2和Nectin-3的正常表达。此外,在第5,7,14,21和28天,隐睾睾丸中乙酰化微管蛋白的模式和排列也发生了变化.隐睾睾丸的超微结构显示精原细胞形成的巨细胞，精母细胞,和圆形和伸长的精子细胞。这项研究的发现表明，隐睾的持续时间与睾丸的异常变化有关，影响生精和支持细胞中蛋白质标记的表达。这些变化源于腹部高温的诱导。
