Abstract：
Spermatogenesis is a highly organized process by which undifferentiated spermatogonia self-renew and differentiate into spermatocytes and spermatids. The entire developmental process from spermatogonia to sperm occurs within the seminiferous tubules. Spermatogenesis is supported by the close interaction of germ cells with Sertoli cells. In this study, testicular tissues were collected from Hu sheep at 8 timepoints after birth: 0, 30, 90, 180, 270, 360, 540, and 720 days. Immunofluorescence staining and histological analysis were used to explore the development of male germ cells and Sertoli cells in the Hu sheep testes at these timepoints. The changes in seminiferous tubule diameter and male germ cells in the Hu sheep testes at these different developmental stages were analyzed. Then, specific molecular markers were used to study the proliferation and differentiation of spermatogonia, the timepoint of spermatocyte appearance, and the maturation and proliferation of Sertoli cells in the seminiferous tubules. Finally, the formation of the blood-testes barrier was studied using antibodies against the main components of the blood-testes barrier, β-catenin, and ZO-1. These findings not only increased the understanding of the development of the Hu sheep testes, but also laid a solid theoretical foundation for Hu sheep breeding.
摘要：
精子发生是未分化的精原细胞自我更新并分化成精母细胞和精子细胞的高度组织化过程。从精原细胞到精子的整个发育过程发生在生精小管内。生殖细胞与支持细胞的紧密相互作用支持精子发生。在这项研究中,在出生后的8个时间点收集湖羊的睾丸组织：0、30、90、180、270、360、540和720天。免疫荧光染色和组织学分析用于探讨这些时间点湖羊睾丸中雄性生殖细胞和支持细胞的发育。分析了湖羊睾丸在这些不同发育阶段的生精管直径和雄性生殖细胞的变化。然后，特异性分子标记用于研究精原细胞的增殖和分化，精母细胞出现的时间点，以及生精小管中支持细胞的成熟和增殖。最后,使用针对血液睾丸屏障主要成分的抗体研究了血液睾丸屏障的形成，β-连环蛋白，ZO-1这些发现不仅增加了对湖羊睾丸发育的认识,同时也为湖羊育种奠定了坚实的理论基础。
