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Abstract：
BACKGROUND: This study evaluated if genetic variations in the WNT family members and RUNX2 are associated with craniofacial maturation, investigating dental and skeletal maturity in children and teenagers.
METHODS: Radiographs from pre-orthodontic treatment of Brazilian patients (7 to 17 years-old) were used to assess dental (panoramic radiographs) and skeletal maturity (cephalometric radiographs). The chronological age (CA) was calculated based on the date of birth and the time the radiographs were performed. For the dental maturity analysis, the Demirjian (1973) method was used and a delta [dental age - chronological age (DA-CA)] was calculated. For the skeletal maturity analysis, the Baccetti et al. (2005) method was used and the patients were classified as \"delayed skeletal maturation\", \"advanced skeletal maturation\" or \"normal skeletal maturation\". DNA isolated from buccal cells was used for genotyping of two genetic variations in WNT family genes: rs708111 (G > A) in WNT3A and rs1533767 (G > A) in WNT11; and two genetic variations in RUNX2: rs1200425 (G > A) and rs59983488 (G > T). A statistical analysis was performed and values of p < 0.05 indicated a significant difference.
RESULTS: There were no associations between dental maturity and genotypes (p > 0.05). In the skeletal maturity analysis, the allele A in the rs708111 (WNT3A) was statistically more frequent in patients with delayed skeletal maturation (Prevalence Ratio = 1.6; 95% Confidence Interval = 1.00 to 2.54; p-value = 0.042).
CONCLUSIONS: The rs708111 in the WNT3A gene impacts on skeletal maturation.
METHODS: Radiographs from pre-orthodontic treatment of Brazilian patients (7 to 17 years-old) were used to assess dental (panoramic radiographs) and skeletal maturity (cephalometric radiographs). The chronological age (CA) was calculated based on the date of birth and the time the radiographs were performed. For the dental maturity analysis, the Demirjian (1973) method was used and a delta [dental age - chronological age (DA-CA)] was calculated. For the skeletal maturity analysis, the Baccetti et al. (2005) method was used and the patients were classified as \"delayed skeletal maturation\", \"advanced skeletal maturation\" or \"normal skeletal maturation\". DNA isolated from buccal cells was used for genotyping of two genetic variations in WNT family genes: rs708111 (G > A) in WNT3A and rs1533767 (G > A) in WNT11; and two genetic variations in RUNX2: rs1200425 (G > A) and rs59983488 (G > T). A statistical analysis was performed and values of p < 0.05 indicated a significant difference.
RESULTS: There were no associations between dental maturity and genotypes (p > 0.05). In the skeletal maturity analysis, the allele A in the rs708111 (WNT3A) was statistically more frequent in patients with delayed skeletal maturation (Prevalence Ratio = 1.6; 95% Confidence Interval = 1.00 to 2.54; p-value = 0.042).
CONCLUSIONS: The rs708111 in the WNT3A gene impacts on skeletal maturation.
摘要：
背景：这项研究评估了WNT家族成员和RUNX2的遗传变异是否与颅面成熟有关，调查儿童和青少年的牙齿和骨骼成熟度。
方法：使用巴西患者（7至17岁）的正畸治疗前的X光片评估牙齿（全景X光片）和骨骼成熟度（头颅X光片）。根据出生日期和进行X射线照相的时间计算实际年龄（CA）。对于牙齿成熟度分析，使用Demirjian（1973）方法，并计算delta[牙齿年龄-实际年龄（DA-CA）]。对于骨骼成熟度分析，Baccetti等人。(2005)方法被使用，患者被归类为“骨骼成熟延迟”，“晚期骨骼成熟”或“正常骨骼成熟”。从口腔细胞分离的DNA用于WNT家族基因中的两个遗传变异的基因分型:WNT3A中的rs708111(G>A)和WNT11中的rs1533767(G>A);以及RUNX2中的两个遗传变异:rs1200425(G>A)和rs59983488(G>T)。进行统计学分析,p<0.05的值指示显著差异。
结果：牙齿成熟度和基因型之间没有相关性（p>0.05）。在骨骼成熟度分析中，rs708111(WNT3A)中的等位基因A在骨骼成熟延迟患者中更常见(患病率比=1.6;95%置信区间=1.00~2.54;p值=0.042).
结论：WNT3A基因中的rs708111影响骨骼成熟。
方法：使用巴西患者（7至17岁）的正畸治疗前的X光片评估牙齿（全景X光片）和骨骼成熟度（头颅X光片）。根据出生日期和进行X射线照相的时间计算实际年龄（CA）。对于牙齿成熟度分析，使用Demirjian（1973）方法，并计算delta[牙齿年龄-实际年龄（DA-CA）]。对于骨骼成熟度分析，Baccetti等人。(2005)方法被使用，患者被归类为“骨骼成熟延迟”，“晚期骨骼成熟”或“正常骨骼成熟”。从口腔细胞分离的DNA用于WNT家族基因中的两个遗传变异的基因分型:WNT3A中的rs708111(G>A)和WNT11中的rs1533767(G>A);以及RUNX2中的两个遗传变异:rs1200425(G>A)和rs59983488(G>T)。进行统计学分析,p<0.05的值指示显著差异。
结果：牙齿成熟度和基因型之间没有相关性（p>0.05）。在骨骼成熟度分析中，rs708111(WNT3A)中的等位基因A在骨骼成熟延迟患者中更常见(患病率比=1.6;95%置信区间=1.00~2.54;p值=0.042).
结论：WNT3A基因中的rs708111影响骨骼成熟。
