Abstract：
BACKGROUND: Many researches have reported the impairment of regulatory T cells (Tregs) in autoimmune hepatitis (AIH), whilst the change of Tregs in peripheral blood remains controversial. We performed this systematic review and meta-analysis to clarify the numerical change of circulating Tregs in AIH patients compared with healthy individuals.
METHODS: Relevant studies were identified from Medline, PubMed, Embase, Web of Science, the Cochrane Library, China National Knowledge Infrastructure, and WanFang Data. Twenty-nine studies involving 968 AIH patients and 583 healthy controls were included. Subgroup analysis stratified by Treg definition or ethnicity was performed, and analysis of active-phase AIH was conducted.
RESULTS: The proportions of Tregs among CD4 T cells and PBMCs were generally decreased in AIH patients compared with healthy controls. Subgroup analysis showed that circulating Tregs identified by CD4+CD25+/high, CD4+CD25+Foxp3+, CD4+CD25+/highCD127-/low, and Tregs in Asian population were decreased among CD4 T cells in AIH patients. No significant change of CD4+CD25+/highFoxp3+CD127-/low Tregs and Tregs in Caucasian population among CD4 T cells were found in AIH patients, whereas the number of studies was limited in these subgroups. Moreover, analysis of the active-phase AIH patients showed that Treg proportions were decreased generally, whereas no significant differences in Tregs/CD4 T cells were observed when markers CD4+CD25+Foxp3+, CD4+CD25+/highFoxp3+CD127-/low were used or in Caucasian population.
CONCLUSIONS: The proportions of Tregs among CD4 T cells and PBMCs were decreased in AIH patients compared with healthy controls generally, whereas Treg definition markers, ethnicity, and disease activity had influence on the results. Further large-scale and rigorous study is warranted.
METHODS: Relevant studies were identified from Medline, PubMed, Embase, Web of Science, the Cochrane Library, China National Knowledge Infrastructure, and WanFang Data. Twenty-nine studies involving 968 AIH patients and 583 healthy controls were included. Subgroup analysis stratified by Treg definition or ethnicity was performed, and analysis of active-phase AIH was conducted.
RESULTS: The proportions of Tregs among CD4 T cells and PBMCs were generally decreased in AIH patients compared with healthy controls. Subgroup analysis showed that circulating Tregs identified by CD4+CD25+/high, CD4+CD25+Foxp3+, CD4+CD25+/highCD127-/low, and Tregs in Asian population were decreased among CD4 T cells in AIH patients. No significant change of CD4+CD25+/highFoxp3+CD127-/low Tregs and Tregs in Caucasian population among CD4 T cells were found in AIH patients, whereas the number of studies was limited in these subgroups. Moreover, analysis of the active-phase AIH patients showed that Treg proportions were decreased generally, whereas no significant differences in Tregs/CD4 T cells were observed when markers CD4+CD25+Foxp3+, CD4+CD25+/highFoxp3+CD127-/low were used or in Caucasian population.
CONCLUSIONS: The proportions of Tregs among CD4 T cells and PBMCs were decreased in AIH patients compared with healthy controls generally, whereas Treg definition markers, ethnicity, and disease activity had influence on the results. Further large-scale and rigorous study is warranted.
摘要：
背景：许多研究报道了自身免疫性肝炎（AIH）中调节性T细胞（Tregs）的损害，而外周血中Tregs的变化仍存在争议。我们进行了系统评价和荟萃分析，以阐明与健康个体相比，AIH患者中循环Tregs的数值变化。
方法：相关研究来自Medline，PubMed,Embase,WebofScience,Cochrane图书馆,中国国家知识基础设施，和万方数据。纳入了29项研究，涉及968例AIH患者和583例健康对照。根据Treg定义或种族进行亚组分析，并对活性相AIH进行了分析。
结果：与健康对照组相比，AIH患者的CD4T细胞和PBMC中Treg的比例普遍降低。亚组分析显示，通过CD4+CD25+/高，CD4+CD25+Foxp3+,CD4+CD25+/高CD127-/低，AIH患者的CD4T细胞中，亚洲人群的Tregs降低。AIH患者CD4T细胞中高加索人群CD4+CD25+/highFoxp3+CD127-/lowTregs和Tregs无明显变化,而这些亚组的研究数量有限.此外，对活跃期AIH患者的分析显示，Treg比例普遍降低，而当标记CD4+CD25+Foxp3+时，Tregs/CD4T细胞没有显着差异，CD4+CD25+/highFoxp3+CD127-/low用于高加索人群。
结论：AIH患者的CD4T细胞和PBMC中Treg的比例与健康对照组相比普遍降低，而Treg定义标记，种族,疾病活动对结果有影响。进一步的大规模和严格的研究是必要的。
方法：相关研究来自Medline，PubMed,Embase,WebofScience,Cochrane图书馆,中国国家知识基础设施，和万方数据。纳入了29项研究，涉及968例AIH患者和583例健康对照。根据Treg定义或种族进行亚组分析，并对活性相AIH进行了分析。
结果：与健康对照组相比，AIH患者的CD4T细胞和PBMC中Treg的比例普遍降低。亚组分析显示，通过CD4+CD25+/高，CD4+CD25+Foxp3+,CD4+CD25+/高CD127-/低，AIH患者的CD4T细胞中，亚洲人群的Tregs降低。AIH患者CD4T细胞中高加索人群CD4+CD25+/highFoxp3+CD127-/lowTregs和Tregs无明显变化,而这些亚组的研究数量有限.此外，对活跃期AIH患者的分析显示，Treg比例普遍降低，而当标记CD4+CD25+Foxp3+时，Tregs/CD4T细胞没有显着差异，CD4+CD25+/highFoxp3+CD127-/low用于高加索人群。
结论：AIH患者的CD4T细胞和PBMC中Treg的比例与健康对照组相比普遍降低，而Treg定义标记，种族,疾病活动对结果有影响。进一步的大规模和严格的研究是必要的。
