Abstract：
OBJECTIVE: Streptococcus parauberis is responsible for the development of streptococcosis in marine fish. The aim of the current study was to determine the antimicrobial susceptibility of aquatic Strep. parauberis strains, thus establishing laboratory-specific epidemiological cut-off (COWT) values to distinguish wild-type (WT) and nonwild-type (NWT) strains.
RESULTS: Using 220 Strep. parauberis isolates obtained from diseased Paralichthys olivaceus, Platichthys stellatus, and Sebastes schlegelii over 6 years from seven different locations in Korea, we established COWT values for eight common antimicrobial agents using the standard broth microdilution method. The COWT values calculated using MIC distribution with the NRI and ECOFFinder methods were the same or within one dilution step for the eight antimicrobials tested. Nine NWT isolates with decreased susceptibility to at least two antimicrobials and one of these isolates exhibited decreased susceptibility to six antimicrobial agents were identified using COWT values based on NRI.
CONCLUSIONS: Interpretive criteria for Strep. parauberis have not yet been established, and the findings of this study provide putative COWT values for eight antimicrobial agents frequently used in aquaculture in Korea.
RESULTS: Using 220 Strep. parauberis isolates obtained from diseased Paralichthys olivaceus, Platichthys stellatus, and Sebastes schlegelii over 6 years from seven different locations in Korea, we established COWT values for eight common antimicrobial agents using the standard broth microdilution method. The COWT values calculated using MIC distribution with the NRI and ECOFFinder methods were the same or within one dilution step for the eight antimicrobials tested. Nine NWT isolates with decreased susceptibility to at least two antimicrobials and one of these isolates exhibited decreased susceptibility to six antimicrobial agents were identified using COWT values based on NRI.
CONCLUSIONS: Interpretive criteria for Strep. parauberis have not yet been established, and the findings of this study provide putative COWT values for eight antimicrobial agents frequently used in aquaculture in Korea.
摘要：
目的：副链球菌是海鱼链球菌病发展的原因。本研究的目的是确定水生副甘草菌株的抗菌敏感性，从而建立实验室特异性流行病学截止值(COWT)以区分野生型(WT)和非野生型(NWT)菌株。
结果：使用从患病的牙牙垢中获得的220株紫草分离物，Platichthysstellatus,和来自韩国七个不同地点的Sebastesschlegelii超过六年，我们使用标准肉汤微量稀释法建立了8种常用抗菌药物的COWT值.使用MIC分布用NRI和ECOFFinder方法计算的COWT值与所测试的八种抗微生物剂相同或在一个稀释步骤内。使用基于NRI的COWT值鉴定出9种对至少两种抗菌剂的敏感性降低的NWT分离株，其中一种对六种抗菌剂的敏感性降低。
结论：对准叶葡萄球菌的解释标准尚未建立，这项研究的结果为韩国水产养殖中常用的八种抗菌剂提供了推定的COWT值。
结果：使用从患病的牙牙垢中获得的220株紫草分离物，Platichthysstellatus,和来自韩国七个不同地点的Sebastesschlegelii超过六年，我们使用标准肉汤微量稀释法建立了8种常用抗菌药物的COWT值.使用MIC分布用NRI和ECOFFinder方法计算的COWT值与所测试的八种抗微生物剂相同或在一个稀释步骤内。使用基于NRI的COWT值鉴定出9种对至少两种抗菌剂的敏感性降低的NWT分离株，其中一种对六种抗菌剂的敏感性降低。
结论：对准叶葡萄球菌的解释标准尚未建立，这项研究的结果为韩国水产养殖中常用的八种抗菌剂提供了推定的COWT值。
