PDF(Pubmed)
Abstract：
Ironwood trees (Casuarina equisetifolia) in Guam have been suffering from Ironwood Tree Decline (IWTD) since 2002. Putative plant pathogenic bacteria such as Ralstonia solanacearum and Klebsiella species were identified in the ooze of declining trees and considered to be linked to IWTD. In addition, termites were found to be significantly associated with IWTD. Microcerotermes crassus Snyder (Blattodea: Termitidae) was identified as a termite species that attacks ironwood trees in Guam. Since termites harbor a diverse community of symbiotic and environmental bacteria, we sequenced the microbiome of M. crassus workers attacking ironwood trees in Guam to assess the presence of IWTD-associated pathogens in termite bodies. This dataset contains 652,571 raw sequencing reads present in M. crassus worker samples collected from six ironwood trees in Guam obtained via sequencing the V4 region of the16S rRNA gene on the Illumina NovaSeq (2 × 250bp) platform. Sequences were taxonomically assigned in QIIME2 using SILVA 132 and NCBI GenBank as reference databases. Spirochaetes and Fibrobacteres were the most dominant phyla in M. crassus workers. No putative plant pathogens of the genera Ralstonia or Klebsiella were found in the M. crassus samples. The dataset has been made publicly available through NCBI GenBank under BioProject ID PRJNA883256. This dataset can be used to compare the bacterial taxa present in M. crassus workers in Guam to bacteria communities of related termite species from other geographical locations. In addition, this dataset can also be used to investigate the relationship between termite microbiomes and the microbiomes of ironwood trees they attack and of the surrounding soil.
摘要：
自2002年以来，关岛的铁木树（木麻黄）一直遭受铁木树下降（IWTD）的困扰。在衰落的树木的软泥中鉴定出了推定的植物病原菌，例如青枯雷尔氏菌和克雷伯氏菌属物种，并被认为与IWTD有关。此外,白蚁被发现与IWTD显著相关。MicrocerotermescrassusSnyder（Blattodea：Termitidae）被确定为攻击关岛铁木树的白蚁物种。由于白蚁拥有不同的共生和环境细菌群落，我们对关岛的M.crassus工人攻击铁木树的微生物组进行了测序，以评估白蚁体内与IWTD相关的病原体的存在。该数据集包含652,571个原始测序读数，这些原始测序读数来自关岛的六棵铁木树，这些样品是通过在IlluminaNovaSeq（2×250bp）平台上对16SrRNA基因的V4区域进行测序获得的。使用SILVA132和NCBIGenBank作为参考数据库在QIIME2中对序列进行分类学分配。螺旋藻和纤维杆菌属是梭鱼工人中最主要的门。在M.crassus样品中未发现Ralstonia或克雷伯氏菌属的推定植物病原体。该数据集已通过NCBIGenBank在BioProjectIDPRJNA883256下公开提供。该数据集可用于将关岛的M.crassus工人中存在的细菌分类群与来自其他地理位置的相关白蚁物种的细菌群落进行比较。此外,该数据集还可用于研究白蚁微生物组与它们攻击的铁木树和周围土壤的微生物组之间的关系。
