Abstract：
In this work, Ti3C2 nano-enzymes (Ti3C2 NEs) materials with simulated peroxidase activity and fluorescence quenching properties were prepared. Then Ti3C2 NEs was functionalized using 6-carboxyfluorescein (FAM) labeled Aflatoxin B1 (AFB1) aptamers to construct a novel multimode nano enzyme biosensor for the detection of AFB1 in peanuts. Based on the fluorescence quenching characteristics and the superior simulated peroxidase activity of Ti3C2 NES and the specific binding of the aptamer to AFB1, the sensitive and rapid fluorescence/colorimetric/smart phone detection of AFB1 have been achieved, with detection limits of 0.09 ng mL-1, 0.61 ng mL-1 and 0.96 ng mL-1, respectively. The analytical method provided can not only detect AFB1 in multiple modes, but also has a wider detection range, lower limit of detection (LOD) and better recovery rate, and can achieve on-site accurate detection of AFB1 content in peanuts, which has great application potential in the field of food quality testing.
摘要：
在这项工作中,制备了具有模拟过氧化物酶活性和荧光猝灭特性的Ti3C2纳米酶(Ti3C2NEs)材料。然后使用6-羧基荧光素（FAM）标记的黄曲霉毒素B1（AFB1）适体对Ti3C2NEs进行功能化，构建了一种新型多模纳米酶生物传感器，用于检测花生中的AFB1。基于Ti3C2NES的荧光猝灭特性和优越的模拟过氧化物酶活性以及适配体与AFB1的特异性结合，实现了AFB1的灵敏、快速的荧光/比色/智能手机检测，检测限分别为0.09ngmL-1、0.61ngmL-1和0.96ngmL-1。提供的分析方法不仅可以在多种模式下检测AFB1，而且检测范围更广，较低的检测限（LOD）和更好的回收率，并能实现花生中AFB1含量的现场准确检测,在食品质量检测领域具有很大的应用潜力。
