Abstract：
The therapeutic efficacy and adverse impacts of nanoparticles (NPs) are strongly dependent on their systemic circulation time. The corona proteins adsorbed on the NPs determine their plasma half-lives, and hence, it is crucial to identify the proteins shortening or extending their circulation time. In this work, the in vivo circulation time and corona composition of superparamagnetic iron oxide nanoparticles (SPIONs) with different surface charges/chemistries were analyzed over time. SPIONs with neutral and positive charges showed the longest and shortest circulation times, respectively. The most striking observation was that corona-coated NPs with similar opsonin/dysopsonin content showed different circulation times, implying these biomolecules are not the only contributing factors. Long-circulating NPs adsorb higher concentrations of osteopontin, lipoprotein lipase, coagulation factor VII, matrix Gla protein, secreted phosphoprotein 24, alpha-2-HS-glycoprotein, and apolipoprotein C-I, while short-circulating NPs adsorb higher amounts of hemoglobin. Therefore, these proteins may be considered to be determining factors governing the NP systemic circulation time.
摘要：
纳米颗粒(NP)的治疗功效和不利影响强烈依赖于它们的体循环时间。NPs上吸附的电晕蛋白决定了它们的血浆半衰期，因此，确定缩短或延长其循环时间的蛋白质至关重要。在这项工作中,随着时间的推移，分析了具有不同表面电荷/化学物质的超顺磁性氧化铁纳米颗粒（SPIONs）的体内循环时间和电晕组成。中性和正电荷的SPION显示出最长和最短的循环时间，分别。最引人注目的观察是，具有相似的调理素/失调素含量的电晕涂层NPs显示出不同的循环时间，暗示这些生物分子不是唯一的促成因素。长循环NPs吸附较高浓度的骨桥蛋白，脂蛋白脂肪酶，凝血因子VII,基质Gla蛋白，分泌磷蛋白24，α-2-HS-糖蛋白，和载脂蛋白C-I,而短循环NPs吸附更大量的血红蛋白。因此,这些蛋白质可能被认为是决定NP体循环时间的决定因素。
