Abstract：
The persistent infection of high-risk-human papillomavirus type 16 (HPV16) is considered an essential element for suffering cervical cancer. Despite polymerase chain reaction, loop-mediated amplification, and microfluidic chips are used to detect the HPV16, these methods still exist some drawbacks including time-consuming and false positive results. The CRISPR-Cas system is widely used in the region of biological detection due to its precise targeted recognition capability. In this contribution, the novel solution-gated graphene transistor sensor is designed to realize the unamplified and label-free detection of HPV16 DNA. Using the precise recognition of the CRISPR-Cas12a system and the gate functionalization, HPV16 DNA can be precisely identified without need the amplification and labeling. The limit of detection of the sensor can be up to 8.3 × 10-18 m and the detection can be within 20 min. Additionally, the heat-Inactivated clinical samples can be clearly distinguished by the sensor the diagnosis results have a high degree of agreement with q-PCR detection.
摘要：
高危型人乳头瘤病毒16型（HPV16）的持续感染被认为是患宫颈癌的基本要素。尽管有聚合酶链反应,环介导扩增，尽管微流控芯片已经用于检测HPV16,但是这些方法仍然存在一些缺点,包括耗时和假阳性结果。CRISPR-Cas系统由于其精确的靶向识别能力而被广泛应用于生物检测领域。在这一贡献中,设计了新型的溶液门控石墨烯晶体管传感器，以实现对HPV16DNA的非扩增和无标记检测。利用CRISPR-Cas12a系统的精确识别和门功能化，无需扩增和标记即可精确鉴定HPV16DNA。传感器的检测限（LOD）可达8.3×10-18M，检测可在20分钟内完成。此外,通过传感器可以清楚地区分热灭活的临床样本，诊断结果与q-PCR检测具有高度的一致性。本文受版权保护。保留所有权利。
