PDF(Pubmed)
Abstract：
Enzymatic terpene functionalization is an essential part of plant secondary metabolite diversity. Within this, multiple terpene-modifying enzymes are required to enable the chemical diversity of volatile compounds essential in plant communication and defense. This work sheds light on the differentially transcribed genes within Caryopteris × clandonensis that are capable of functionalizing cyclic terpene scaffolds, which are the product of terpene cyclase action. The available genomic reference was subjected to further improvements to provide a comprehensive basis, where the number of contigs was minimized. RNA-Seq data of six cultivars, Dark Knight, Grand Bleu, Good as Gold, Hint of Gold, Pink Perfection, and Sunny Blue, were mapped on the reference, and their distinct transcription profile investigated. Within this data resource, we detected interesting variations and additionally genes with high and low transcript abundancies in leaves of Caryopteris × clandonensis related to terpene functionalization. As previously described, different cultivars vary in their modification of monoterpenes, especially limonene, resulting in different limonene-derived molecules. This study focuses on predicting the cytochrome p450 enzymes underlying this varied transcription pattern between investigated samples. Thus, making them a reasonable explanation for terpenoid differences between these plants. Furthermore, these data provide the basis for functional assays and the verification of putative enzyme activities.
摘要：
酶促萜烯官能化是植物次生代谢产物多样性的重要组成部分。在这里面,需要多种萜烯修饰酶来实现植物通讯和防御所必需的挥发性化合物的化学多样性。这项工作揭示了Caryopteris×clandonensis中的差异转录基因，这些基因能够功能化环状萜烯支架，这是萜烯环化酶作用的产物。现有的基因组参考进行了进一步的改进，以提供全面的基础，其中重叠群的数量被最小化。六个品种的RNA-Seq数据，黑暗骑士,大布鲁，像黄金一样好，黄金的提示，粉红完美，还有阳光蓝,被映射到引用上，并研究了它们独特的转录谱。在此数据资源中，我们在与萜烯官能化有关的Caryopteris×clandonensis的叶子中检测到了有趣的变异以及具有高和低转录丰度的基因。如前所述，不同的品种对单萜的修饰不同，尤其是柠檬烯,产生不同的柠檬烯衍生分子。这项研究的重点是预测所研究样品之间这种变化的转录模式的细胞色素p450酶。因此，使它们成为这些植物之间萜类化合物差异的合理解释。此外，这些数据为功能测定和验证推定的酶活性提供了基础。
