PDF(Pubmed)
Abstract：
The elevation of the snowline of the No. 1 Glacier in the Tianshan Mountains is increasing due to global warming, which has created favorable conditions for moss invasion and offers an opportunity to investigate the synergistic effects of incipient succession by mosses, plants, and soils. In this study, the concept of altitude distance was used instead of succession time. To investigate the changes of bacterial-community diversity in moss-covered soils during glacial degeneration, the relationship between bacterial community structure and environmental factors was analyzed and valuable microorganisms in moss-covered soils were explored. To do so, the determination of soil physicochemical properties, high-throughput sequencing, the screening of ACC-deaminase-producing bacteria, and the determination of ACC-deaminase activity of strains were performed on five moss-covered soils at different elevations. The results showed that the soil total potassium content, soil available phosphorus content, soil available potassium content, and soil organic-matter content of the AY3550 sample belt were significantly different compared with those of other sample belts (p < 0.05). Secondly, there was a significant difference (p < 0.05) in the ACE index or Chao1 index between the moss-covered-soil AY3550 sample-belt and the AY3750 sample-belt bacterial communities as the succession progressed. The results of PCA analysis, RDA analysis, and cluster analysis at the genus level showed that the community structure of the AY3550 sample belt and the other four sample belts differed greatly and could be divided into two successional stages. The enzyme activities of the 33 ACC-deaminase-producing bacteria isolated and purified from moss-covered soil at different altitudes ranged from 0.067 to 4.7375 U/mg, with strains DY1-3, DY1-4, and EY2-5 having the highest enzyme activities. All three strains were identified as Pseudomonas by morphology, physiology, biochemistry, and molecular biology. This study provides a basis for the changes in moss-covered soil microhabitats during glacial degradation under the synergistic effects of moss, soil, and microbial communities, as well as a theoretical basis for the excavation of valuable microorganisms under glacial moss-covered soils.
摘要：
编号雪线的海拔。1由于全球变暖，天山冰川正在增加，这为苔藓入侵创造了有利条件，并为研究苔藓早期演替的协同效应提供了机会，植物,和土壤。在这项研究中,使用高度距离的概念代替了连续时间。为研究苔藓覆盖土壤冰川退化过程中细菌群落多样性的变化,分析了苔藓覆盖土壤中细菌群落结构与环境因子的关系，探索了有价值的微生物。要做到这一点，土壤理化性质的测定，高通量测序，ACC脱氨酶产生菌的筛选,并在不同海拔的五种苔藓覆盖土壤上测定了菌株的ACC脱氨酶活性。结果表明,土壤全钾含量,土壤有效磷含量，土壤有效钾含量，AY3550样带的土壤有机质含量与其他样带相比差异显着（p<0.05）。其次,随着演替的进行，苔藓覆盖土壤AY3550样品带和AY3750样品带细菌群落之间的ACE指数或Chao1指数存在显着差异（p<0.05）。PCA分析的结果，RDA分析,属水平的聚类分析表明，AY3550样带与其他四个样带的群落结构差异很大，可分为两个演替阶段。从不同海拔高度的苔藓覆盖土壤中分离纯化的33种产生ACC脱氨酶的细菌的酶活性范围为0.067至4.7375U/mg，其中菌株DY1-3、DY1-4和EY2-5具有最高的酶活性。通过形态学鉴定三株均为假单胞菌,生理学,生物化学,和分子生物学。本研究为苔藓协同作用下冰川退化过程中苔藓覆盖土壤微生境的变化提供了依据,土壤,和微生物群落，以及在冰川苔藓覆盖的土壤中挖掘有价值的微生物的理论基础。
