PDF(Pubmed)
Abstract：
Bats may carry various viruses and bacteria which can be harmful to humans, but little is known about their role as a parasitic source with zoonotic potential. The aim of this study was to test wild bats for the presence of selected parasites: Toxoplasma gondii, Neospora caninum and microsporidia Encephalitozoon spp. In total, brain and small intestine tissues of 100 bats (52 Myotis myotis, 43 Nyctalus noctula and 5 Vespertilio murinus) were used for the DNA isolation and PCR detection of the abovementioned agents. Toxoplasma gondii DNA was detected by real-time PCR in 1% of bats (in one male of M. myotis), while all bats were negative for N. caninum DNA. Encephalitozoon spp. DNA was detected by nested PCR in 25% of bats, including three species (twenty-two M. myotis, two N. noctula and one V. murinus). Positive samples were sequenced and showed homology with the genotypes Encephalitozoon cuniculi II and Encephalitozoon hellem 2C. This is the first study on wild vespertilionid bats from Central Europe and worldwide, with a relatively high positivity of Encephalitozoon spp. detected in bats.
摘要：
蝙蝠可能携带各种对人类有害的病毒和细菌，但是人们对它们作为具有人畜共患潜力的寄生源的作用知之甚少。这项研究的目的是测试野生蝙蝠是否存在选定的寄生虫：弓形虫，犬新孢子虫和小孢子虫。总的来说,100只蝙蝠的大脑和小肠组织(52只肌炎，43Nyctalusnoctula和5Vespertiliomurinus）用于上述试剂的DNA分离和PCR检测。通过实时PCR在1％的蝙蝠中检测到弓形虫DNA（在一个雄性的M.myotis），而所有的蝙蝠都是阴性的。头孢菌素属。25％的蝙蝠通过巢式PCR检测到DNA，包括三个物种(22个M.myotis,两个N.noctula和一个V.murinus)。对阳性样品进行了测序，并显示出与基因型头孢菌素II和头孢菌素类2C的同源性。这是对来自中欧和全世界的野生蛇麻蝙蝠的首次研究，具有相对较高的头孢菌素阳性。在蝙蝠中发现。
