PDF(Pubmed)
Abstract：
In this study, we investigated the properties of human varicose vein (VV) endothelial cells (HVVEC) in comparison to the human umbilical vein endothelial cells (HUVEC). The cells were treated with three bioactive compounds with proven beneficial effects in the therapy of patients with VV, diosmin, escin, and bromelain. Two concentrations of tested drugs were used (1, 10 mg/mL), which did not affect the viability of either cell type. Escin led to a slight generation of reactive oxygen species in HUVEC cells. We observed a slight release of superoxide in HVVEC cells upon treatment with diosmin and escin. Diosmin and bromelain showed a tendency to release nitric oxide in HUVEC. Using membrane fluorescent probes, we demonstrated a reduced fluidity of HVVEC, which may lead to their increased adhesion, and, consequently, a much more frequent occurrence of venous thrombosis. For the first time, we show the mechanism of action of drugs used in VV therapy on endothelial cells derived from a VV. Studies with HVVEC have shown that tested drugs may lead to a reduction in the adhesive properties of these cells, and thus to a lower risk of thrombosis.
摘要：
在这项研究中,我们研究了人静脉曲张（VV）内皮细胞（HVVEC）与人脐静脉内皮细胞（HUVEC）的特性。用三种生物活性化合物治疗细胞，这些化合物在治疗VV患者中具有良好的效果，Diosmin,escin,还有菠萝蛋白酶.使用两种浓度的测试药物（1,10mg/mL），这不影响任何一种细胞类型的活力。Escin导致HUVEC细胞中活性氧的轻微产生。我们观察到用地奥司明和七叶皂苷处理后，HVVEC细胞中超氧化物的轻微释放。Diosmin和菠萝蛋白酶在HUVEC中显示出释放一氧化氮的趋势。使用膜荧光探针，我们证明了HVVEC的流动性降低，这可能会导致它们的附着力增加，and,因此,静脉血栓形成更频繁的发生。第一次,我们显示了用于VV治疗的药物对VV衍生的内皮细胞的作用机制。HVVEC的研究表明，测试药物可能导致这些细胞的粘附特性降低，从而降低血栓形成的风险。
