PDF(Pubmed)
Abstract：
In recent years, various forms of caloric restriction (CR) and amino acid or protein restriction (AAR or PR) have shown not only success in preventing age-associated diseases, such as type II diabetes and cardiovascular diseases, but also potential for cancer therapy. These strategies not only reprogram metabolism to low-energy metabolism (LEM), which is disadvantageous for neoplastic cells, but also significantly inhibit proliferation. Head and neck squamous cell carcinoma (HNSCC) is one of the most common tumour types, with over 600,000 new cases diagnosed annually worldwide. With a 5-year survival rate of approximately 55%, the poor prognosis has not improved despite extensive research and new adjuvant therapies. Therefore, for the first time, we analysed the potential of methionine restriction (MetR) in selected HNSCC cell lines. We investigated the influence of MetR on cell proliferation and vitality, the compensation for MetR by homocysteine, the gene regulation of different amino acid transporters, and the influence of cisplatin on cell proliferation in different HNSCC cell lines.
摘要：
近年来,各种形式的热量限制（CR）和氨基酸或蛋白质限制（AAR或PR）不仅在预防与年龄相关的疾病方面取得了成功，比如II型糖尿病和心血管疾病，还有癌症治疗的潜力。这些策略不仅将新陈代谢重新编程为低能量代谢（LEM），这对肿瘤细胞是不利的，而且还显著抑制增殖。头颈部鳞状细胞癌（HNSCC）是最常见的肿瘤类型之一，全球每年诊断出超过60万例新病例。5年生存率约为55%，尽管进行了广泛的研究和新的辅助治疗,但不良预后并未得到改善.因此,第一次,我们分析了选定的HNSCC细胞系中蛋氨酸限制（MetR）的潜力。我们研究了MetR对细胞增殖和活力的影响，高半胱氨酸对MetR的补偿，不同氨基酸转运蛋白的基因调控,以及顺铂对不同HNSCC细胞系细胞增殖的影响。
