PDF(Pubmed)
Abstract：
Antimicrobial susceptibility testing plays a pivotal role in the discovery of new antibiotics. However, the development of simple, sensitive, and rapid assessment approaches remains challenging. Herein, we report an activated alkyne-based cascade signal amplification strategy for ultrafast and high-throughput antibiotic screening. First of all, a novel water-soluble aggregation-induced emission (AIE) luminogen is synthesized, which contains an activated alkyne group to enable fluorescence turn-on and metal-free click bioconjugation under physiological conditions. Taking advantage of the in-house established method for bacterial lysis, a number of clickable biological substances (i.e., bacterial solutes and debris) are released from the bacterial bodies, which remarkably increases the quantity of analytes. By means of the activated alkyne-mediated turn-on click bioconjugation, the system fluorescence signal is significantly amplified due to the increased labeling sites as well as the AIE effect. Such a cascade signal amplification strategy efficiently improves the detection sensitivity and thus enables ultrafast antimicrobial susceptibility assessment. By integration with a microplate reader, this approach is further applied to high-throughput antibiotic screening.
摘要：
抗菌药物敏感性试验在新抗生素的发现中起着举足轻重的作用。然而,简单的发展，敏感,快速评估方法仍然具有挑战性。在这里，我们报道了一种基于激活炔烃的级联信号放大策略,用于超快和高通量抗生素筛选.首先,合成了一种新型的水溶性聚集诱导发射（AIE）发光体，其含有活化的炔基,以使得能够在生理条件下进行荧光开启和无金属点击生物缀合。利用内部建立的细菌裂解方法，许多可点击的生物物质(即，细菌溶质和碎片)从细菌体内释放，这显著增加了分析物的数量。通过激活的炔烃介导的开启点击生物缀合，由于增加的标记位点以及AIE效应,系统荧光信号被显著放大。这种级联信号放大策略有效地提高了检测灵敏度,因此能够进行超快的抗微生物敏感性评估。通过与酶标仪集成，该方法进一步应用于高通量抗生素筛选.
