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Abstract：
Hubei mosquito virus 2 (HMV2) is a novel mosquito virus that was first identified in 2016 in Hubei Province, China. Until now, HMV2 has been shown to be endemic in some areas of China and Japan, but its biological characteristics, epidemiology, and pathogenicity are not yet known. This report describes the detection of HMV2 in mosquitoes that were collected in Shandong Province in 2019 and presents the first isolation and molecular characterization of the virus. In this study, a total of 2,813 mosquitoes were collected and then divided into 57 pools, according to location and species. qRT-PCR and nested PCR were performed to confirm the presence of HMV2, and its genomic features, phylogenetic relationships, growth characteristics, and potential pathogenicity were further analyzed. The results showed that HMV2 was detected in 28 of the 57 mosquito pools and that the minimum infection rate (MIR) for HMV2 was 1.00% (28/2,813). A HMV2 strain and 14 viral partial sequences were obtained from the HMV2-positive pools, including one complete genome sequence. A phylogenetic analysis revealed that HMV2 from Shandong Province shared over 90% identity with other reported isolates and was closely related to the Culex inatomii luteo-like virus. IMPORTANCE Our study provided important epidemiological evidence for the epidemic of HMV2 in Shandong Province. Here, we report the first isolation and molecular characteristics of this virus and enrich our knowledge of the distribution of HMV2 in China.
摘要：
湖北蚊子病毒2型(HMV2)是湖北省2016年首次发现的新型蚊子病毒,中国。直到现在,HMV2已被证明在中国和日本的某些地区是地方性的，但是它的生物学特性，流行病学,致病性尚不清楚。本报告描述了2019年在山东省收集的蚊子中HMV2的检测，并介绍了该病毒的首次分离和分子特征。在这项研究中,共收集了2813只蚊子，然后分成57个水池，根据位置和物种。进行qRT-PCR和巢式PCR以确认HMV2的存在及其基因组特征，系统发育关系，生长特性，并进一步分析了潜在的致病性。结果表明，在57个蚊子池中的28个中检测到HMV2，HMV2的最低感染率（MIR）为1.00％（28/2,813）。从HMV2阳性库获得HMV2株和14个病毒部分序列，包括一个完整的基因组序列。系统发育分析表明，来自山东省的HMV2与其他报告的分离株具有90％以上的同一性，并且与库蚊黄样病毒密切相关。重要性我们的研究为山东省HMV2的流行提供了重要的流行病学证据。这里,我们报道了该病毒的首次分离和分子特征，并丰富了我们对HMV2在中国的分布的了解。
