Abstract：
The primary aim of this study was to assess the histomorphometric outcomes of extraction sockets grafted with freeze-dried bone allograft (FDBA) and sealed with a collagen membrane after 3 months of healing in specific region of interest (ROI) areas. The secondary aims were to analyze the biomaterial resorption rate, the bone-to-biomaterial contact (BBC), and the area and perimeter of grafted particles compared with commercially available FDBA particles. Fifteen patients underwent tooth extractions and ridge preservation procedures performed with FDBA and a collagen membrane. Bone biopsy samples were harvested after 3 months at the time of implant placement for histologic and histomorphometric analysis. Two areas of concern (ROI1 and ROI2) with different histologic features were identified within the biopsy samples; ROI1, ROI2, and commercially available particles were analyzed and compared. The following parameters were analyzed: newly formed bone, marrow space, residual graft particles, perimeter and area of FDBA particles, and BBC. The histomorphometric analysis showed 35.22% ± 10.79% newly formed bone, 52.55% ± 16.06% marrow spaces, and 12.41% ± 7.87% residual graft particles. Moreover, the histologic data from ROI1 and ROI2 showed that (1) the mean percentage of BBC was 64.61% ± 27.14%; (2) the newly formed bone was significantly higher in ROI1 than in ROI2; (3) the marrow space was significantly lower in ROI1 than in ROI2; and (4) the FDBA particles in ROI1 sites showed significantly lower area and perimeter when compared to commercially available FDBA particles. This latter data led to the hypothesis that FDBA particles embedded in newly formed bone undergo a resorption/remodeling process.
摘要：
这项研究的主要目的是评估在ROI（感兴趣区域）区域愈合3个月后，用冻干同种异体骨移植（FDBA）移植并用胶原膜密封的拔牙槽的组织形态计量学结果。次要目的是分析生物材料的吸收率，与市售的FDBA颗粒相比，骨与生物材料的接触以及移植颗粒的面积/周长。15例患者接受了用FDBA和胶原膜进行的拔牙和牙沟保存手术。在植入物插入3个月后收集骨活检用于组织学和组织形态计量学分析。在活检样本中鉴定了具有不同组织学特征的两个关注区域ROI1和ROI2;分析并比较了ROI1、ROI2和市售颗粒。分析了以下参数：新形成的骨（NFB），骨髓间隙(MS),残余接枝颗粒(RGP)，FDBA颗粒和骨与生物材料接触的周长/面积。组织形态学分析显示新形成的骨占35.22±10.79%，骨髓间隙的52.55±16.06％和残余移植物的12.41±7.87％。此外，来自感兴趣区域（ROI）1和2的组织学数据表明：a）骨与生物材料接触（BBC）的平均百分比为64.61±27.14％；b）ROI1中的新形成骨明显高于ROI2；c）ROI1中的骨髓空间明显低于ROI2和d）ROI1区域中的FDBA颗粒与FDBA相比，其周长明显较低。后面的数据导致以下假设:嵌入在新形成的骨中的FBA颗粒经历再吸收/重塑过程。
