Abstract：
Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most important diseases threatening wheat production both in Turkey and worldwide. However, the underlying genetic dynamics of Pst populations are not fully known in Turkey. To determine the population genetic structure and migration network among regional Pst populations, a total of 140 Pst isolates collected from six geographical regions of Turkey from 2018 to 2020 were sampled and genotyped using 21 simple sequence repeat loci. A total of 70 multilocus genotypes were identified and classified into the three major genetic groups by Bayesian assignment. The highest genotypic diversity was detected in Southeastern Anatolia, showing its critical role as one of the source populations to trigger possible stripe rust epidemics. Analysis of molecular variance revealed the highest variation (90.25%) within isolates. The migration network generated by the number of effective migrants showed that the highest migration (1.0) was determined between Southeastern Anatolia and Central Anatolia, and considerable levels of migration (>0.2) were determined among the other regions, except for the Black Sea. Linkage equilibrium (P ≥ 0.05) was detected for many geographical regions, except for Marmara (P = 0.00) and the Mediterranean (P = 0.03), suggesting that reproduction of Pst populations is most likely sexual or mixed (sexual and clonal). To sum up, this is the first study on the genetic relationships and population genetic structure of the Pst population in Turkey, and these findings may provide critical information to develop management strategies for wheat stripe rust.
摘要：
条锈病,由条锈病引起。小麦（Pst），是威胁土耳其和全世界小麦生产的最重要疾病之一。然而,土耳其尚未完全了解Pst种群的潜在遗传动态。为了确定区域Pst种群之间的种群遗传结构和迁移网络，从2018年至2020年从土耳其的6个地理区域收集的总共140个Pst分离株进行了采样,并使用21个简单序列重复位点进行了基因分型.总共鉴定了70个多位点基因型，并通过贝叶斯分配将其分为三个主要的遗传组。在安纳托利亚东南部检测到最高的基因型多样性，显示出其作为源种群之一的关键作用，以引发可能的条锈病流行。分子方差分析显示分离株内变异最高(90.25%)。由有效移民数量产生的移民网络表明，在安纳托利亚东南部和安纳托利亚中部之间确定了最高的移民（1.0），在其他地区确定了相当大的迁移水平(>0.2)，除了黑海。在许多地理区域检测到联动平衡(P≥0.05)，除了马尔马拉（P=0.00）和地中海（P=0.03），表明Pst种群的繁殖很可能是有性的或混合的（有性和克隆的）。总而言之,这是对土耳其Pst种群的遗传关系和种群遗传结构的首次研究，这些发现可能为制定小麦条锈病的管理策略提供关键信息。
