PDF(Pubmed)
Abstract：
Implementing hemoprotozoan control strategies in dogs has become difficult because of the co-infections. A multiplex polymerase chain reaction (PCR) was carried out for simultaneous detection of the co-infections of Babesia gibsoni, B. vogeli, Hepatozoon canis and Ehrlichia canis from dogs (N = 442) in Andhra Pradesh, South India. The co-infection combinations were classified as (i) B. gibsoni + B. vogeli + E. canis + H. canis (BEH), (ii) B. gibsoni + B. vogeli + E. canis (BE), (iii) B. gibsoni + B. vogeli + H. canis (BH) and (iv) E. canis + H. canis (EH) groups. The parasite-specific multiplex PCR amplified 18S rRNA gene of B. gibsoni, B. vogeli and H. canis and VirB9 gene of E. canis. The age, gender, breed, medium, living condition and region of dogs were studied as risk factors for co-infections using logistic regression model. Among the co-infections, the incidence was 1.81%, 9.28%, 0.69% and 0.90% for BEH, BE, BH and EH infections, respectively. Young age (< one year), females, mongrels, rural dogs, kennel dogs and presence of ticks were the identified risk factors for overall prevalence of tick-borne pathogens. The incidence of infection was less in rainy season, especially in dogs with a previous acaricidal treatment. The study concludes that the multiplex PCR assay could simultaneously detect natural co-infections in dogs, emphasizing the need for the assay in epidemiological studies to reveal the real pattern of pathogens and select pathogen-specific treatment protocols.
摘要：
由于共同感染，在狗中实施原生动物控制策略变得困难。进行了多重聚合酶链反应（PCR），以同时检测双歧杆菌的共感染，B.Vogeli,安得拉邦的狗（N=442）的肝虫犬和埃里希氏菌犬，南印度。合并感染的组合被分类为(i)B.gibsoni+B.vogeli+E.canis+H.canis(BEH),(ii)B.gibsoni+B.vogeli+E.canis(BE)，(iii)B.gibsoni+B.vogeli+H.canis(BH)和(iv)E.canis+H.canis(EH)基团。寄生虫特异性多重PCR扩增双歧杆菌18SrRNA基因,犬B.vogeli和H.canis和E.canis的VirB9基因。年龄,性别,品种,中等,使用logistic回归模型研究了狗的生存状况和区域作为共同感染的危险因素。在共感染中，发病率为1.81%,9.28%，BEH为0.69%和0.90%，BE,BH和EH感染，分别。年龄（<1岁），女性,杂鼠,农村的狗,犬舍犬和蜱的存在是蜱传病原体总体流行的确定危险因素。雨季感染发生率较低，尤其是在以前进行过杀螨剂治疗的狗中。该研究得出结论，多重PCR检测可以同时检测狗的自然共感染，强调需要在流行病学研究中进行分析，以揭示病原体的真实模式并选择特定病原体的治疗方案。
