PDF(Pubmed)
Abstract：
Streptococcus gallolyticus subspecies gallolyticus (SGG) and Fusobacterium (F.) nucleatum have been implicated in colorectal carcinogenesis. Here, the association of immune responses to bacterial exposure with advancing stages of colorectal neoplasia was assessed by multiplex serology.
Immunoglobulin (Ig) A and G antibody responses to eleven proteins each of F. nucleatum and SGG were measured in plasma of controls (n = 100) and patients with colorectal cancer (CRC, n = 25), advanced adenoma (n = 82), or small polyps (n = 85). Multivariable logistic regression was used to evaluate the association of bacterial sero-positivity with colorectal neoplasia. In a cohort subset with matched data (n = 45), F. nucleatum sero-positivity was correlated with bacterial abundance in both neoplastic and matched normal tissue.
IgG sero-positivity to Fn1426 of F. nucleatum was associated with an increased CRC risk (OR = 4.84; 95% CI 1.46-16.0), while IgA sero-positivity to any SGG protein or specifically Gallo0272 and Gallo1675 alone was associated with increased advanced adenoma occurrence (OR = 2.02, 95% CI 1.10-3.71; OR = 2.67, 95% CI 1.10-6.46; and OR = 6.17, 95% CI 1.61-23.5, respectively). Only F. nucleatum abundance in the normal mucosa positively correlated with the IgA response to the Fn1426 antigen (Correlation coefficient (r) = 0.38, p < 0.01).
Antibody responses to SGG and F. nucleatum were associated with occurrence of colorectal adenomas and CRC, respectively. Further studies are needed to clarify the role these microbes or the immune response to their antigens may have in colorectal carcinogenesis stages.
Immunoglobulin (Ig) A and G antibody responses to eleven proteins each of F. nucleatum and SGG were measured in plasma of controls (n = 100) and patients with colorectal cancer (CRC, n = 25), advanced adenoma (n = 82), or small polyps (n = 85). Multivariable logistic regression was used to evaluate the association of bacterial sero-positivity with colorectal neoplasia. In a cohort subset with matched data (n = 45), F. nucleatum sero-positivity was correlated with bacterial abundance in both neoplastic and matched normal tissue.
IgG sero-positivity to Fn1426 of F. nucleatum was associated with an increased CRC risk (OR = 4.84; 95% CI 1.46-16.0), while IgA sero-positivity to any SGG protein or specifically Gallo0272 and Gallo1675 alone was associated with increased advanced adenoma occurrence (OR = 2.02, 95% CI 1.10-3.71; OR = 2.67, 95% CI 1.10-6.46; and OR = 6.17, 95% CI 1.61-23.5, respectively). Only F. nucleatum abundance in the normal mucosa positively correlated with the IgA response to the Fn1426 antigen (Correlation coefficient (r) = 0.38, p < 0.01).
Antibody responses to SGG and F. nucleatum were associated with occurrence of colorectal adenomas and CRC, respectively. Further studies are needed to clarify the role these microbes or the immune response to their antigens may have in colorectal carcinogenesis stages.
摘要：
背景：溶胆囊链球菌亚种（SGG）和梭杆菌属（F.)核仁与结直肠癌的发生有关。这里,通过多重血清学评估了免疫应答与细菌暴露与结直肠肿瘤进展期的相关性.
方法：在对照组（n=100）和结直肠癌患者（CRC，n=25),晚期腺瘤（n=82），或小息肉（n=85）。多变量logistic回归用于评估细菌血清阳性与结直肠肿瘤的相关性。在具有匹配数据的队列子集中(n=45)，F.核仁血清阳性与肿瘤和匹配的正常组织中的细菌丰度相关。
结果：核仁F.的Fn1426IgG血清阳性与CRC风险增加相关（OR=4.84；95％CI1.46-16.0），而IgA对任何SGG蛋白或特别是Gallo0272和Gallo1675的血清阳性与晚期腺瘤发生增加相关(OR=2.02,95%CI1.10-3.71;OR=2.67,95%CI1.10-6.46;和OR=6.17,95%CI1.61-23.5)。正常粘膜中只有F.核仁丰度与IgA对Fn1426抗原的反应呈正相关(相关系数(r)=0.38,p<0.01)。
结论：SGG和核囊F.的抗体反应与结直肠腺瘤和CRC的发生有关，分别。需要进一步的研究来阐明这些微生物或对其抗原的免疫反应可能在结直肠癌发生阶段中的作用。
方法：在对照组（n=100）和结直肠癌患者（CRC，n=25),晚期腺瘤（n=82），或小息肉（n=85）。多变量logistic回归用于评估细菌血清阳性与结直肠肿瘤的相关性。在具有匹配数据的队列子集中(n=45)，F.核仁血清阳性与肿瘤和匹配的正常组织中的细菌丰度相关。
结果：核仁F.的Fn1426IgG血清阳性与CRC风险增加相关（OR=4.84；95％CI1.46-16.0），而IgA对任何SGG蛋白或特别是Gallo0272和Gallo1675的血清阳性与晚期腺瘤发生增加相关(OR=2.02,95%CI1.10-3.71;OR=2.67,95%CI1.10-6.46;和OR=6.17,95%CI1.61-23.5)。正常粘膜中只有F.核仁丰度与IgA对Fn1426抗原的反应呈正相关(相关系数(r)=0.38,p<0.01)。
结论：SGG和核囊F.的抗体反应与结直肠腺瘤和CRC的发生有关，分别。需要进一步的研究来阐明这些微生物或对其抗原的免疫反应可能在结直肠癌发生阶段中的作用。
