PDF(Pubmed)
Abstract：
Plants cope with various recurring stress conditions that often induce DNA damage, ultimately affecting plant genome integrity, growth, and productivity. The CROWDED NUCLEI (CRWN) family comprises lamin-like proteins with multiple functions, such as regulating gene expression, genome organization, and DNA damage repair in Arabidopsis (Arabidopsis thaliana). However, the mechanisms and consequences of CRWNs in DNA damage repair are largely unknown. Here, we reveal that CRWNs maintain genome stability by forming repairing nuclear bodies at DNA double-strand breaks. We demonstrate that CRWN1 and CRWN2 physically associate with the DNA damage repair proteins RAD51D and SUPPRESSOR OF NPR1-1 Inducible 1 (SNI1) and act in the same genetic pathway to mediate this process. Moreover, CRWN1 and CRWN2 partially localize at γ-H2AX foci upon DNA damage. Notably, CRWN1 and CRWN2 undergo liquid-liquid phase separation to form highly dynamic droplet-like structures with RAD51D and SNI1 to promote the DNA damage response (DDR). Collectively, our data shed light on the function of plant lamin-like proteins in the DDR and maintenance of genome stability.
摘要：
植物应对经常引起DNA损伤的各种反复出现的胁迫条件，最终影响植物基因组的完整性，增长和生产力。CROWDEDNUCLEI(CRWN)家族包含具有多种功能的层粘连蛋白样蛋白,比如调节基因表达，拟南芥的基因组组织和DNA损伤修复。然而,CRWNs在DNA损伤修复中的作用机制和后果尚不清楚.这里,我们发现CRWN通过在DNA双链断裂时形成修复核体来维持基因组稳定性。我们证明CRWN1和CRWN2与DNA损伤修复蛋白RAD51D和NPR1-1诱导型1（SNI1）的抑制蛋白物理相关，并在相同的遗传途径中发挥作用以介导这一过程。此外，CRWN1和CRWN2在DNA损伤后部分定位于γ-H2AX灶。值得注意的是,CRWN1和CRWN2经过液-液相分离，与RAD51D和SNI1形成高度动态的液滴状结构，以促进DNA损伤反应（DDR）。总的来说，我们的数据揭示了植物层粘连蛋白样蛋白在DDR中的功能和维持基因组稳定性。
