PDF(Pubmed)
Abstract：
Flaxseed/linseed is an important oilseed crop having applications in the food, nutraceutical, and paint industry. Seed weight is one of the most crucial determinants of seed yield in linseed. Here, quantitative trait nucleotides (QTNs) associated with thousand-seed weight (TSW) have been identified using multi-locus genome-wide association study (ML-GWAS). Field evaluation was carried out in five environments in multi-year-location trials. SNP genotyping information of the AM panel of 131 accessions comprising 68,925 SNPs was employed for ML-GWAS. From the six ML-GWAS methods employed, five methods helped identify a total of 84 unique significant QTNs for TSW. QTNs identified in ≥ 2 methods/environments were designated as stable QTNs. Accordingly, 30 stable QTNs have been identified for TSW accounting up to 38.65% trait variation. Alleles with positive effect on trait were analyzed for 12 strong QTNs with r 2 ≥ 10.00%, which showed significant association of specific alleles with higher trait value in three or more environments. A total of 23 candidate genes have been identified for TSW, which included B3 domain-containing transcription factor, SUMO-activating enzyme, protein SCARECROW, shaggy-related protein kinase/BIN2, ANTIAUXIN-RESISTANT 3, RING-type E3 ubiquitin transferase E4, auxin response factors, WRKY transcription factor, and CBS domain-containing protein. In silico expression analysis of candidate genes was performed to validate their possible role in different stages of seed development process. The results from this study provide significant insight and elevate our understanding on genetic architecture of TSW trait in linseed.
摘要：
亚麻籽/亚麻籽是一种重要的油料作物，在食品中具有重要的应用，营养食品，和油漆工业。种子重量是亚麻籽种子产量最关键的决定因素之一。这里,已使用多基因座全基因组关联研究（ML-GWAS）鉴定了与千粒重（TSW）相关的定量性状核苷酸（QTNs）。在多年定位试验中，在五个环境中进行了实地评估。包含68,925个SNP的131个种质的AM组的SNP基因分型信息用于ML-GWAS。从采用的六种ML-GWAS方法中，五种方法帮助鉴定了总共84个独特的TSW显著QTNs。在≥2种方法/环境中鉴定的QTNs被指定为稳定的QTNs。因此，已鉴定出TSW的30个稳定QTNs，占38.65％的性状变异。对性状有积极影响的等位基因分析了12个强QTNs，r2≥10.00%，这表明特定等位基因在三个或更多环境中具有较高性状值的显着关联。总共有23个候选基因被鉴定为TSW，其中包括含有B3结构域的转录因子，SUMO激活酶，蛋白质切row，shaggy相关蛋白激酶/BIN2,抗生物素蛋白3,环型E3泛素转移酶E4,生长素反应因子,WRKY转录因子,和含有CBS结构域的蛋白质。进行了候选基因的计算机表达分析，以验证它们在种子发育过程的不同阶段中的可能作用。这项研究的结果提供了重要的见解，并提高了我们对亚麻籽TSW性状遗传结构的理解。
