Abstract：
While pioneering methods have demonstrated that bacterial N-acyl homoserine lactone (AHL) signaling molecules can influence the growth and self-aggregation of suspended microalgae, whether AHLs can affect the initial adhesion to a carrier has remained an open question. Here we revealed that the microalgae exhibited different adhesion potential under AHL mediation, where the performance was affiliated to both AHL types and concentrations. The result can be well explained by the interaction energy theory, where the energy barrier between the carriers and the cells varied due to AHL mediation. Depth analyses revealed that AHL acted through modifying the properties of the surface electron donor of the cells, which were dependent upon three major components, i.e., extracellular protein (PN) secretion, the PN secondary structure, and the PN amino acid composition. These findings expand the known diversity of AHLs mediation on microalgal initial adhesion and metabolisms, which may interface with other major cycles and become helpful to theoretically guide the application of AHLs in microalgal culture and harvesting.
摘要：
虽然开创性的方法已经证明细菌N-酰基高丝氨酸内酯（AHL）信号分子可以影响悬浮微藻的生长和自聚集，AHL是否会影响对载体的初始粘附力仍然是一个悬而未决的问题。在这里，我们发现微藻在AHL介导下表现出不同的粘附潜力，性能与AHL类型和浓度有关。结果可以用相互作用能理论很好地解释，由于AHL的介导，载体和细胞之间的能垒发生变化。深度分析表明，AHL通过改变细胞表面电子供体的性质起作用，这取决于三个主要组成部分，即，细胞外蛋白（PN）分泌，PN二级结构，和PN氨基酸组成。这些发现扩大了AHLs介导微藻初始粘附和代谢的已知多样性，这可能与其他主要循环相联系，有助于理论指导AHLs在微藻培养和收获中的应用。
