Fifty patients, aged between 18-80 years, who were diagnosed with allergic asthma and 18 healthy volunteers were included in the study. After the collection 2 mL of total blood from volunteers, RNA isolation and cDNA synthesis were performed. For miRNA profile screening, expression analysis was performed by real-time PCR method using miScript miRNA PCR Array. GeneGlobe Data Analysis Center was used to evaluate dysregulated miRNAs.
In the allergic asthma group, 9 (18%) of the patients were male and 41 (82%) of them were female. In the control group, 7 (38.89%) were male and 11 (61.1%) were female (P:0.073). As a result of the research, the expression levels of miR-142-5p, miR-376c-3p and miR-22-3p were down-regulated, while miR-27b-3p, miR-26b-5p, miR-15b-5p and miR-29c-3p detected as up-regulated.
The results of our study suggest that miR142-5p, miR376c-3p and miR22-3p promote Ubiquitin-mediated proteolysis by inhibiting TGF-β expression through a mechanism involving the p53 signaling pathway. The deregulated miRNAs may be used as a diagnostic and prognostic biomarker in asthma.
■50名患者,年龄在18-80岁之间,被诊断为过敏性哮喘的患者和18名健康志愿者被纳入研究.从志愿者身上采集两毫升总血液后,进行RNA分离和cDNA合成。对于miRNA谱筛选,通过使用miScriptmiRNAPCR阵列的实时PCR方法进行表达分析。GeneGlobe数据分析中心用于评估失调的miRNA。
■在过敏性哮喘组中,9例(18%)患者为男性,41例(82%)为女性。在对照组中,男性7例(38.89%),女性11例(61.1%)(P:0.073)。作为研究的结果,miR-142-5p的表达水平,miR-376c-3p和miR-22-3p下调,而miR-27b-3p,miR-26b-5p,miR-15b-5p和miR-29c-3p被检测为上调。
■我们的研究结果表明miR142-5p,miR376c-3p和miR22-3p通过涉及p53信号通路的机制抑制TGF-β表达促进泛素介导的蛋白水解。失调的miRNA可用作哮喘的诊断和预后生物标志物。